Date published: 2025-9-9

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LAMP-2 항체 (M3/84): sc-19991

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데이터 시트
  • LAMP-2 항체 M3/84 는 랫드 monoclonal IgG1, 60간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • 면역 흡착제 정제 mouse 대식세포 당단백질 분획에 대한 항원 결정기
  • 안티-LAMP-2 항체 (M3/84)는 WB, IP, IF, IHC(P) and FCM으로 mouse, rat and human유래의 LAMP-2 를 감지하는 데에 추천한다.
  • 항-LAMP-2 항체(M3/84)는 IP용 agarose와 결합되어 이용 가능하며, WB, IHC(P), ELISA용 HRP와 결합되어 이용 가능하며, IF, IHC(P), FCM용 phycoerythrin 또는 FITC와 결합되어 이용 가능합니다.
  • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • LAMP-2 (M3/84): sc-19991무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • 현재 LAMP-2 항체(M3/84)에 대해 선호하는 2차 검출 시약의 식별을 아직 완료하지 못했습니다. 이 작업은 진행 중입니다.

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LAMP-2 항체(M3/84)는 WB, IP, IF, IHC(P) 및 FCM에 의해 마우스, 랫 및 인간 기원의 LAMP-2를 검출하는 IgG1 랫 모노클로날 LAMP-2 항체(또한 LAMP-2 항체 또는 LAMP2 항체로 지정됨)이다. LAMP-2 항체(M3/84)는 비 conjug 결합 항-LAMP-2 항체 형태뿐만 아니라 아가로오스, HRP, PE, FITC 및 다중 Alexa Fluor® 접합체를 포함한 다중 접합 형태의 항-LAMP-2 항체로 사용할 수 있다. 리소좀 관련 막 단백질(LAMP)은 색소 멜라닌의 생합성에 역할을 하는 글리코실화된 I형 막 단백질이다. LAMP-1(또한 지정된 CD107A) 및 LAMP-2(또한 지정된 CD107B)는 세포 부착을 포함한 다양한 기능에 관여하며 종양 침입 및 전이 과정에 참여하는 것으로 판단된다. 새로 합성된 LAMP-1 및 LAMP-2 단백질은 트랜스 골지 네트워크에서 분류되고 만노스-6 인산염 수용체에 사용되는 클라트린 코팅된 소포와 구별되는 경로를 통해 세포 내로 수송된다. LAMP-1은 트롬빈 활성화된 혈소판의 표면에 발현되지만 휴지 혈소판은 발현되지 않으며, 이들 세포의 접착제, 프로트롬브 특성에 관여하는 것으로 판단된다. LAMP-1 및 LAMP-2는 모두 리소좀 산도를 유지하고 리소좀 막을 자가소화로부터 보호하는 데 관여하며, 리소좀 저장 장애 환자에서 그 발현이 증가한다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

주문정보

제품명카탈로그 번호 단위가격수량관심품목

LAMP-2 항체 (M3/84)

sc-19991
200 µg/ml
$316.00

LAMP-2 항체 (M3/84) AC

sc-19991 AC
500 µg/ml, 25% agarose
$416.00

LAMP-2 항체 (M3/84) HRP

sc-19991 HRP
200 µg/ml
$316.00

LAMP-2 항체 (M3/84) FITC

sc-19991 FITC
200 µg/ml
$330.00

LAMP-2 항체 (M3/84) PE

sc-19991 PE
200 µg/ml
$343.00

LAMP-2 항체 (M3/84) Alexa Fluor® 488

sc-19991 AF488
200 µg/ml
$357.00

LAMP-2 항체 (M3/84) Alexa Fluor® 546

sc-19991 AF546
200 µg/ml
$357.00

LAMP-2 항체 (M3/84) Alexa Fluor® 594

sc-19991 AF594
200 µg/ml
$357.00

LAMP-2 항체 (M3/84) Alexa Fluor® 647

sc-19991 AF647
200 µg/ml
$357.00

LAMP-2 항체 (M3/84) Alexa Fluor® 680

sc-19991 AF680
200 µg/ml
$357.00

LAMP-2 항체 (M3/84) Alexa Fluor® 790

sc-19991 AF790
200 µg/ml
$357.00

What epitope is this LAMP-2 (Antibody (M3/84): sc-19991) antibody raised against? This question was asked previously *but the wrong antibody is referenced in responding to this question. LAMP-2 is absolutely a transmembrane protein.*

Asked by: Julian Yano
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801ext.1, allowing for a more interactive discussion of this and other related questions.
Answered by: Blake J
Date published: 2021-11-23

What is the epitope this Ab was raised against? Is it lumenal or cytoplasmic?

Asked by: Julian Yano
Thank you for your question. Anti-STC1 Antibody (1A3): sc-293435 was raised against amino acids 141-247 representing partial length STC1 of human origin. It is a secreted protein, so there is no cytoplasmic domain.
Answered by: Tech Support Europe
Date published: 2021-11-22

Can LAMP-2 (M3/84): sc-19991 be used to IHQ (not paraffin-embedded) from frozen tissue sections?

Asked by: yarasi
Thank you for your question. Yes, this antbody is recommended and warrantied for IF and for IHC on fresh frozen tissue sections.
Answered by: Tech Service
Date published: 2019-12-09

Hallo What secondary antibodies can be used with this product?

Asked by: Soll
Thank you for your question. You can use any secondary antibody that is specific against rat IgG1 antibodies to detect LAMP-2 Antibody (M3/84): sc-19991. For further information please contact our technical service directly.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

Can LAMP-2 (M3/84): sc-19991 be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenni
Thank you for your question. Yes, LAMP-2 (M3/84): sc-19991 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 15
Date published: 2017-02-07
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Rated 4 out of 5 by from Works OkayThe Anti-LAMP2 works okay (not great) for paraffin mice brain section section IF staining after Citrate EDTA pH6.2 HIER for 20 minutes. IT does work a bit better in the floating frozen sections of mouse brain without any antigen retrieval. Need to troubleshoot the signal in paraffin section though
Date published: 2021-12-13
Rated 3 out of 5 by from Lots of noisy background with this antibody forLots of noisy background with this antibody for murine cells.
Date published: 2019-12-04
Rated 5 out of 5 by from wb5%milk blocked, diluted in 1:500, the band is clear,and the backgroud is clean
Date published: 2017-12-13
Rated 5 out of 5 by from Lots of signalThere was a strong band at 120 KDa in mouse aorta and NIH/3T3 samples by WB
Date published: 2017-08-11
Rated 5 out of 5 by from Very popular for IF/IHC(P)!This rat monoclonal antibody, clone (M3/84) is a very popular choice for IF and IHC(P) applications for detection of LAMP-2 in mouse, rat and human samples.
Date published: 2017-01-24
Rated 4 out of 5 by from Used in WBUsed in WB, worked fine.
Date published: 2015-07-30
Rated 3 out of 5 by from Large smear observed by Western Blot using J774ALarge smear observed by Western Blot using J774A.1 whole cell lysate. -SCBT QC
Date published: 2015-04-04
Rated 5 out of 5 by from Two publications cite the use of this antibodyTwo publications cite the use of this antibody with mouse aorta and NIH/3T3 samples, analyzed by Western Blot. -SCBT Publication Review
Date published: 2015-03-27
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LAMP-2 항체 (M3/84) is rated 4.1 out of 5 by 9.
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