Date published: 2025-9-9

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Lamin B1 항체 (B-10): sc-374015

4.5(53)
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데이터 시트
  • Lamin B1 항체(B-10) 는 마우스 monoclonal IgG1κ, 314간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • 항원결정부는 mouse origin의 Lamin B11의 내부에 위치한 559-586의 아미노산에 해당됩니다.
  • 안티-Lamin B1 항체 (B-10)는 WB, IP, IF and ELISA으로 mouse, rat and human유래의 Lamin B1 를 감지하는 데에 추천한다.
  • 항-Lamin B1 항체(B-10)는 IP용 agarose와 결합되어 이용 가능하며, WB, IHC(P), ELISA용 HRP와 결합되어 이용 가능하며, IF, IHC(P), FCM용 phycoerythrin 또는 FITC와 결합되어 이용 가능합니다.
  • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • Lamin B1 (B-10): sc-374015무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgG Fc BP-HRPm-IgGκ BP-HRP는 Lamin B1 항체 (B-10) WB 애플리케이션용입니다. 이 시약은 현재 Lamin B1 항체 (B-10)(아래 주문 정보 참조)와 함께 번들로 제공됩니다.

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Lamin B1 항체(B-10)는 마우스, 쥐 및 인간 유래의 Lamin B1을 WB, IP, IF 및 ELISA로 검출하는 IgG1 κ 마우스 모노클로날 Lamin B1 항체입니다. Lamin B1 항체(B-10)는 비접합 항-Lamin B1 항체 형태뿐만 아니라 아가로스, HRP, PE, FITC 및 여러 Alexa Fluor® 접합체를 포함한 여러 접합 형태의 항-Lamin B1 항체 형태로도 사용할 수 있습니다. 이전에 설명한 프로테아제 계열과는 서열, 구조 및 기질 특이성이 다른 독특한 시스테인 프로테아제 계열이 설명되었습니다. Ced-3/ICE라고 불리는 이 계열은 세포 사멸 기계의 핵심 구성 요소로 기능하며 세포 수명에 중요한 특정 표적 단백질을 파괴하는 작용을 합니다. 핵 라민은 핵 외피의 틀을 제공하고 염색질과 상호 작용할 수 있는 것으로 생각되는 핵 내막의 핵질 쪽 섬유층인 핵 라미나의 구성 요소로서 핵 외피의 완전성과 세포 형태를 유지하는 데 중요합니다. B형 라민은 파네실화 및 인산화와 같은 일련의 변형을 거칩니다. 라민의 인산화 증가는 외피 붕괴 전에 발생하며 아마도 라민 결합을 조절하는 역할을 할 것입니다. 핵 라민 B는 미확인된 ICE 계열의 구성원에 의한 세포 사멸의 결과로 단편화됩니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

주문정보

제품명카탈로그 번호 단위가격수량관심품목

Lamin B1 항체 (B-10)

sc-374015
200 µg/ml
$316.00

Lamin B1 (B-10): m-IgG Fc BP-HRP 번들

sc-529634
200 µg Ab; 10 µg BP
$354.00

Lamin B1 (B-10): m-IgGκ BP-HRP 번들

sc-522588
200 µg Ab, 40 µg BP
$354.00

Lamin B1 항체 (B-10) AC

sc-374015 AC
500 µg/ml, 25% agarose
$416.00

Lamin B1 항체 (B-10) HRP

sc-374015 HRP
200 µg/ml
$316.00

Lamin B1 항체 (B-10) FITC

sc-374015 FITC
200 µg/ml
$330.00

Lamin B1 항체 (B-10) PE

sc-374015 PE
200 µg/ml
$343.00

Lamin B1 항체 (B-10) Alexa Fluor® 488

sc-374015 AF488
200 µg/ml
$357.00

Lamin B1 항체 (B-10) Alexa Fluor® 546

sc-374015 AF546
200 µg/ml
$357.00

Lamin B1 항체 (B-10) Alexa Fluor® 594

sc-374015 AF594
200 µg/ml
$357.00

Lamin B1 항체 (B-10) Alexa Fluor® 647

sc-374015 AF647
200 µg/ml
$357.00

Lamin B1 항체 (B-10) Alexa Fluor® 680

sc-374015 AF680
200 µg/ml
$357.00

Lamin B1 항체 (B-10) Alexa Fluor® 790

sc-374015 AF790
200 µg/ml
$357.00

Lamin B1 (B-10) 중화펩타이드

sc-374015 P
100 µg/0.5 ml
$68.00

Lamin B1 抗体 (B-10) sc-374015について。 200μg/mlで、量は何マイクロリットルでしょうか?

Asked by: okada
ご質問ありがとうございます。体積は1mlとなります。宜しくお願い致します。 Thank you for your question. The volume is 1 ml. Thank you for your cooperation.
Answered by: Jiajun Z
Date published: 2024-07-03

I will do fluorescent WB from subcellular fractionation. which Lamin B is suitable for me for nucleus HK?

Asked by: Medania
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801ext.1, allowing for a more interactive discussion of this and other related questions.
Answered by: Blake J
Date published: 2021-11-23

Hi, do the directly labelled antibodies still have the Fc part. Indeed, one aim to use them would be to use another antibody made in mouse simultaneously in IF

Asked by: Clairec
Thank you for your question. Anti-Lamin B1 Antibody (B-10): sc-374015 is a complete mouse monoclonal IgG1 antibody with both Fab and Fc regions.
Answered by: Tech Support Europe
Date published: 2021-04-28

Before using this Ab, dose the extraction of nuclear proteins require specific steps for efficient detection of lamin B1

Asked by: jaou
Thank you for your question. You can follow our recommended protocol, which can be found under this link: https://www.scbt.com/de/resources/protocols/western-immuno-blotting
Answered by: Technical Support Europe
Date published: 2020-10-28

I used to do ChIP by using anti-LaminB (M-20) sc-6217 with Dynabeads protein G, and it worked well. Which magnetic beads do you recommend for ChIP using Lamin B1 Antibody (B-10): sc-374015 ?

Asked by: Naoko
Thank you for your question. This Lamin B1 antibody should work with any anti-mouse IgG magnetic beads. Since you have protein G magnetic beads, this should work fine since protein G has a strong binding to mouse IgG1. If you have any further questions, please contact our Asia Technical Service team. You can reach them by phone at (86 21) 6093-6350, by email at: asia@scbio.cn or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-09-05

I'm trying to use this Ab for IF in dermal fibroblasts, so far with no results. I use methanol fixation at -20C for 5 min, 0,3% triton RT 3min, FBS blocking for 30 min and primary Ab incubation at 4C ON. Should I do anything different for it to work?

Asked by: Sofiamp
Thank you for your question. A link to our recommended protocol for IF using this mouse monoclonal antibody, Lamin B1 (B-10): sc-374015, is provided below. We recommend using the primary antibody at a starting dilution of 1:50. If after consulting our protocol you continue to have negative results, please contact our Technical Service Department for help with troubleshooting the antibody. Please call our European Support office toll-free at 00800 4573 8000, email europe@scbt.com, or contact us by live chat. https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-11-21

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with Lamin B1 Antibody (B-10): sc-374015?

Asked by: cjMara
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 10
Date published: 2017-01-09
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Rated 5 out of 5 by from Works great for WBWorks well on neonatal mouse lungs at concentration of 30ug. Antibody dilution will be 1:500 for overnight at 4oC
Date published: 2024-12-13
Rated 5 out of 5 by from Good for WBA clear band around the target size is detected in the nuclear isolation sample.
Date published: 2024-03-27
Rated 5 out of 5 by from Great for WBThe antibody is good for western blot. The band is single and clear.
Date published: 2024-03-19
Rated 5 out of 5 by from Ottimo funzionamento in IF!Ho provato questo anticorpo in immunofluorescenza e funziona molto bene con una marcatura definita a livello della membrana nucleare
Date published: 2023-11-30
Rated 5 out of 5 by from Clear Band in Western BlotsThe antibody worked nicely in Western Blot analyses of human cell lysates using dilutions at 1:500-1:1000.
Date published: 2023-11-25
Rated 4 out of 5 by from Works nicely for WBIt works nicely in total cellular lysates and fractionation experiments in MCF-7 cells.
Date published: 2023-10-18
Rated 4 out of 5 by from Good antibodyWorked well with western blotting at 1:500 dilution.
Date published: 2023-04-14
Rated 4 out of 5 by from Good workingI successfully detected murine Lamin B, but not human cells (A549).
Date published: 2023-04-13
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Lamin B1 항체 (B-10) is rated 4.5 out of 5 by 53.
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