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Lamin A/C 항체 (346): sc-7293

4.5(12)
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데이터 시트
  • Lamin A/C 항체(346) 는 마우스 monoclonal IgMκ, 147간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • rat B 림프모구 세포주 SKW6.4에 대해 양성 반응
  • WB, IP와 IF으로 mouse, rat와 human origin의 Lamin A and Lamin C을 검출할것을 권장합니다.
  • IP에 사용가능한 agarose결합제품 이 있습니다.
  • See Lamin A/C (E-1): sc-376248 for additional antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • Lamin A/C (346): sc-7293무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • 현재 Lamin A/C 항체(346)에 대해 선호하는 2차 검출 시약의 식별을 아직 완료하지 못했습니다. 이 작업은 진행 중입니다.

빠른 링크

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Lamin A/C 항체(346)는 WB, IP 및 IF에 의해 마우스, 랫트 및 인간 기원의 Lamin A 및 Lamin C를 검출하는 IgM κ 마우스 단일 클론 Lamin A/C 항체이다. Lamin A/C 항체(346)는 공액 및 비공액 항-Lamin A/C 항체 형태로 모두 사용할 수 있다. 이전에 설명된 어떤 단백질분해효소 계열과도 서열, 구조 및 기질 특이성이 다른 독특한 시스테인 단백질분해효소 계열이 설명되었다. Ced-3/ICE로 명명된 이 계열은 ICE, CPP32, ICH-1/Nedd-2, Tx, Mch2, Mch3(ICE-LAP3 또는 CMH-1), Mch4 및 ICE-LAP6으로 구성된다. Ced-3/ICE 계열 구성체는 세포자멸사 기계의 핵심 구성 요소로 기능하고 세포 수명에 중요한 특정 표적 단백질을 파괴하는 작용을 한다. 핵 라민은 핵 외피와 세포 형태의 무결성을 유지하는 데 중요하다. 핵 라민 A는 Mch2에 의해 절단되지만 CPP32는 절단되지 않는다. 핵 라민 B는 ICE 계열의 미확인 구성체에 의한 세포 자멸의 결과로 단편화된다. 라민 C는 라민 A의 스플라이스 변형으로, 카르복시 말단에서만 다르다. 라민 A와 C는 처음 566개 아미노산에서 동일하며, 라민 C는 6개의 고유한 카르복시 말단 아미노산에서만 다르다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

주문정보

제품명카탈로그 번호 단위가격수량관심품목

Lamin A/C 항체 (346)

sc-7293
200 µg/ml
$316.00

Lamin A/C 항체 (346) AC

sc-7293 AC
500 µg/ml, 25% agarose
$416.00

Will the m-IgGk BP-HRP (sc-516102-CM) recognize the IgM kappa light chain of this Lamin A/C antibody? If not, which secondary antibody is suitable?

Asked by: WB_user
Thank you for your question. Yes, a mouse IgG binding protein will be able to pick up this IgM antibody. Unless you are using the Cruz Markers, you do not need to be using the CM form of the secondary (CM stands for Cruz Marker compatible). You can find our available secondaries here: https://www.scbt.com/scbt/browse/Secondary-Antibodies/_/N-1hoaxk4
Answered by: Tech Service
Date published: 2019-08-14

Was this antibody also used for FACS? Were there good results?

Asked by: AmitB
Thank you for your question. We do not recommend Lamin A/C Antibody (346): sc-7293 for the detection of Lamin A/C in flow cytometry. However a different antibody, Lamin A/C Antibody (E-1): sc-376248, is available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for flow cytometry. Lamin A/C Antibody (636): sc-7292 is available conjugated to Alexa Fluor® 594, Alexa Fluor® 680, or Alexa Fluor® 790 for flow cytometry.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

请问LAMIN A/C抗体 (346)中的346是什么意思?这个抗体能检测到pre-laminA/C吗?谢谢!

Asked by: fenping zheng
感谢您的提问。346是这支单克隆抗体的克隆名,sc-7293 Lamin A/C (346)推荐检测 Lamin A and Lamin C。 Thank you for your question. 346 is the clone name of this monoclonal antibody.sc-7293 Lamin A/C (346) is recommended for detection of Lamin A and Lamin C
Answered by: Technical Support 8
Date published: 2018-12-21

Does this antibody recognize progerin? What was the region it was raised against? Thank you

Asked by: Jéssica Sousa
Thank you for your question. This antibody is specific for Lamin A/C. It was raised against nuclear lamin fraction of rat liver origin, the exact epitope has not been mapped. If you have any further questions, please contact Technical Service.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-15

Hello! I have one question about your sc-7293 lamin A/C antibody, do you know which epitope this antibody targets? Thank you!

Asked by: Yizhi Jiang
Thank you for your question. Lamin A/C Antibody (346) is a mouse monoclonal IgM raised against nuclear lamin fraction of rat liver origin. The exact epitope recognized by this antibody has not been determined.
Answered by: Technical Support
Date published: 2017-03-01

Is Lamin A/C Antibody (346): sc-7293 available directly conjugated to HRP or fluorescent dyes?

Asked by: AbPolly
Thank you for your question. All requests for directly conjugated versions of this antibody should be directed to our Special Orders team at specialorders@scbt.com Alternatively, consider using Lamin A/C (E-1): sc-376248 for Lamin A/C antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647 (https://www.scbt.com/scbt/product/lamin-a-c-antibody-e-1)
Answered by: Technical Support 3
Date published: 2017-01-09
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Rated 1 out of 5 by from Not suitable for mouse IFI compared E-1 (sc-376248) and 346 (sc-7293) for IF of both MEF wt and MEF Lmna-/-. 346 is not capable of IF with mouse embryonic fibroblasts in our condition (4%PFA 15min fixation followed 1:500 dilution / 4C overnight reaction).
Date published: 2021-03-07
Rated 4 out of 5 by from Lamin A/C anti-mouse antibodyI used this antibody as a control for nuclear fraction of my study and came out good.
Date published: 2018-02-22
Rated 5 out of 5 by from nice and clear bandIt worked quite good in WB for canine samples. Double band around 70 kDa
Date published: 2017-11-06
Rated 5 out of 5 by from Works great!!I got it few weeks back. I used it at 1:1000 dilution, O/N incubation at 4 C. It worked just awesome like they have in their datasheet.
Date published: 2017-08-10
Rated 5 out of 5 by from Great WBVery nice western blot results; also IF staining data looks good
Date published: 2017-01-11
Rated 5 out of 5 by from Strong Signal, Good for WBThis antibody is well-cited for a variety of applications including WB, IP, and IF. Strong, clear signal with little to no background. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2017-01-05
Rated 5 out of 5 by from Use this antibody in Western BlotUse this antibody in Western Blot. Great signal.
Date published: 2015-10-20
Rated 5 out of 5 by from Great monoclonal antibodyGreat monoclonal antibody! Specific staining in CCD-1064Sk and FHs 173We whole cell lysates. -SCBT QC
Date published: 2015-04-15
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Lamin A/C 항체 (346) is rated 4.5 out of 5 by 12.
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