IRF3 Antibody (SL-12): sc-33641
- IRF3 Antibody (SL-12) is a mouse monoclonal IgG1 κ IRF3 antibody, cited in 86 publications, provided at 200 µg/ml
- raised against recombinant IRF-3 fusion protein corresponding to human IRF-3 (amino acids 56-427)
- IRF3 Antibody (SL-12) is recommended for detection of IRF-3 of mouse, rat and human origin by WB, IP, IF and IHC(P)
- Anti-IRF3 Antibody (SL-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for Gel Supershift and ChIP applications (sc-33641 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for IRF3 Antibody (SL-12) for WB and IHC(P) applications. These reagents are now offered in bundles with IRF3 Antibody (SL-12) (see ordering information below).
IRF-3 Antibody (SL-12) is a mouse monoclonal IgG1 kappa light chain antibody designed to target the Interferon Regulatory Factor 3 (IRF-3) protein with exceptional precision. Engineered against a recombinant IRF-3 fusion protein corresponding to human IRF-3 (amino acids 56-427), anti-IRF-3 antibody (SL-12) reliably detects IRF-3 from mouse, rat, and human species across a variety of applications, including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). IRF-3, also referred to as IIAE7, is a critical transcription factor in the innate immune response, playing a vital role in the detection of viral infections and the subsequent activation of type I interferons, such as IFN-β. Upon viral infection, IRF-3 undergoes phosphorylation, dimerizes, and translocates to the nucleus where IRF-3 binds to specific DNA elements to initiate the transcription of interferon genes, thereby orchestrating a robust antiviral defense mechanism. Understanding and analyzing IRF-3′s function is essential for elucidating the molecular pathways involved in immune regulation and the body′s response to pathogens. IRF-3 (SL-12) monoclonal antibody is available in both non-conjugated form and a variety of conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatile tools for researchers to investigate the intricate roles of IRF-3 in cellular processes and disease states.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
IRF3 Antibody (SL-12) References:
- Triggering the interferon response: the role of IRF-3 transcription factor. | Hiscott, J., et al. 1999. J Interferon Cytokine Res. 19: 1-13. PMID: 10048763
- Overlapping and distinct mechanisms regulating IRF-3 and IRF-7 function. | Servant, MJ., et al. 2002. J Interferon Cytokine Res. 22: 49-58. PMID: 11846975
- Control of IRF-3 activation by phosphorylation. | Yoneyama, M., et al. 2002. J Interferon Cytokine Res. 22: 73-6. PMID: 11846977
- Identification and characterization of MAVS, a mitochondrial antiviral signaling protein that activates NF-kappaB and IRF 3. | Seth, RB., et al. 2005. Cell. 122: 669-82. PMID: 16125763
- The kinase complex responsible for IRF-3-mediated IFN-beta production in myeloid dendritic cells (mDC). | Sasai, M., et al. 2006. J Biochem. 139: 171-5. PMID: 16452304
- Distinct functions of IRF-3 and IRF-7 in IFN-alpha gene regulation and control of anti-tumor activity in primary macrophages. | Solis, M., et al. 2006. Biochem Pharmacol. 72: 1469-76. PMID: 16846591
- IRF-3-dependent and augmented target genes during viral infection. | Andersen, J., et al. 2008. Genes Immun. 9: 168-75. PMID: 18094709
- The roles of IRF-3 and IRF-7 in innate antiviral immunity against dengue virus. | Chen, HW., et al. 2013. J Immunol. 191: 4194-201. PMID: 24043884
- Thymoquinone Suppresses IRF-3-Mediated Expression of Type I Interferons via Suppression of TBK1. | Aziz, N., et al. 2018. Int J Mol Sci. 19: PMID: 29751576
- The Structural Basis of IRF-3 Activation upon Phosphorylation. | Jing, T., et al. 2020. J Immunol. 205: 1886-1896. PMID: 32826280
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IRF3 Antibody (SL-12) | sc-33641 | 200 µg/ml | $322.00 | |||
IRF3 Antibody (SL-12): m-IgG Fc BP-HRP Bundle | sc-526695 | 200 µg Ab; 10 µg BP | $361.00 | |||
IRF3 Antibody (SL-12): m-IgG1 BP-HRP Bundle | sc-532068 | 200 µg Ab; 20 µg BP | $361.00 | |||
IRF3 Antibody (SL-12) X | sc-33641 X | 200 µg/0.1 ml | $322.00 | |||
IRF3 Antibody (SL-12) AC | sc-33641 AC | 500 µg/ml, 25% agarose | $424.00 | |||
IRF3 Antibody (SL-12) HRP | sc-33641 HRP | 200 µg/ml | $322.00 | |||
IRF3 Antibody (SL-12) FITC | sc-33641 FITC | 200 µg/ml | $336.00 | |||
IRF3 Antibody (SL-12) PE | sc-33641 PE | 200 µg/ml | $349.00 | |||
IRF3 Antibody (SL-12) Alexa Fluor® 488 | sc-33641 AF488 | 200 µg/ml | $364.00 | |||
IRF3 Antibody (SL-12) Alexa Fluor® 546 | sc-33641 AF546 | 200 µg/ml | $364.00 | |||
IRF3 Antibody (SL-12) Alexa Fluor® 594 | sc-33641 AF594 | 200 µg/ml | $364.00 | |||
IRF3 Antibody (SL-12) Alexa Fluor® 647 | sc-33641 AF647 | 200 µg/ml | $364.00 | |||
IRF3 Antibody (SL-12) Alexa Fluor® 680 | sc-33641 AF680 | 200 µg/ml | $364.00 | |||
IRF3 Antibody (SL-12) Alexa Fluor® 790 | sc-33641 AF790 | 200 µg/ml | $364.00 |