IRF-2 Antibody (G-10): sc-374327

IRF-2 Antibody (G-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects IRF-2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). IRF-2 (G-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. IRF-2 protein plays a crucial role in regulating immune responses, particularly in type I interferon signaling. IRF-2 protein is primarily located in the nucleus, where IRF-2 functions as a transcriptional regulator. Nuclear localization allows IRF-2 protein to interact directly with DNA and modulate interferon-inducible gene expression. DNA binding capability within promoters of these genes is essential for maintaining balance between immune activation and repression, critical for preventing excessive inflammation and autoimmunity. IRF-2 protein′s interaction with other interferon regulatory factor family members, such as IRF-1, highlights IRF-2′s importance in immune regulation networks, where IRF-2 can either promote or inhibit gene expression depending on cellular context.

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IRF-2 Antibody (G-10) References:

1. Activation and repression of the 2-5A synthetase and p21 gene promoters by IRF-1 and IRF-2.  |  Coccia, EM., et al. 1999. Oncogene. 18: 2129-37. PMID: 10321737
2. The role of the interferon regulatory factors, IRF-1 and IRF-2, in LPS-induced cyclooxygenase-2 (COX-2) expression in vivo and in vitro.  |  Zhang, S., et al. 2002. J Endotoxin Res. 8: 379-88. PMID: 12537697
3. Involvement of IFN regulatory factor (IRF)-1 and IRF-2 in the formation and progression of human esophageal cancers.  |  Wang, Y., et al. 2007. Cancer Res. 67: 2535-43. PMID: 17363571
4. IRF-2 regulates NF-kappaB activity by modulating the subcellular localization of NF-kappaB.  |  Chae, M., et al. 2008. Biochem Biophys Res Commun. 370: 519-24. PMID: 18395009
5. SUMOylated IRF-1 shows oncogenic potential by mimicking IRF-2.  |  Park, SM., et al. 2010. Biochem Biophys Res Commun. 391: 926-30. PMID: 19962964
6. Structurally similar but functionally distinct factors, IRF-1 and IRF-2, bind to the same regulatory elements of IFN and IFN-inducible genes.  |  Harada, H., et al. 1989. Cell. 58: 729-39. PMID: 2475256
7. Evidence for a nuclear factor(s), IRF-1, mediating induction and silencing properties to human IFN-beta gene regulatory elements.  |  Fujita, T., et al. 1988. EMBO J. 7: 3397-405. PMID: 2850164
8. Recognition DNA sequences of interferon regulatory factor 1 (IRF-1) and IRF-2, regulators of cell growth and the interferon system.  |  Tanaka, N., et al. 1993. Mol Cell Biol. 13: 4531-8. PMID: 7687740
9. Cellular commitment to oncogene-induced transformation or apoptosis is dependent on the transcription factor IRF-1.  |  Tanaka, N., et al. 1994. Cell. 77: 829-39. PMID: 8004672
10. The oncogenic transcription factor IRF-2 possesses a transcriptional repression and a latent activation domain.  |  Yamamoto, H., et al. 1994. Oncogene. 9: 1423-8. PMID: 8152803
11. Jak-STAT pathways and transcriptional activation in response to IFNs and other extracellular signaling proteins.  |  Darnell, JE., et al. 1994. Science. 264: 1415-21. PMID: 8197455
12. Transcription factors IRF-1 and IRF-2: linking the immune responses and tumor suppression.  |  Taniguchi, T. 1997. J Cell Physiol. 173: 128-30. PMID: 9365509

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-IRF-2 Antibody (G-10) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-IRF-2 Antibody (G-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

IRF-2 Antibody (G-10) Specifications

Alternate Product Name: IRF-2

Product Clone Name: G-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 317-346

Epitope Target: IRF-2

Epitope Species: human

Product Reactivity/Detection: IRF-2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine and bovine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for IRF-2 Antibody (G-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, X: TransCruz Reagent