ING4 항체 (B-8) WB, IP, IF와 ELISA으로 mouse, rat와 human origin의 ING4 and ING5을 검출할것을 권장합니다.

ING4 항체 (B-8): sc-515714

ING4 항체 (B-8) is rated 5.0 out of 5 by 1.
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데이터 시트

    • ING4 항체(B-8) 는 마우스 monoclonal IgG1(kappa light chain) 이며 200 µg/ml으로 제공합니다.
    • human origin의 ING4 and ING5에서 에 가까운90-109의 아미노산사이에 위치한 항원결정부와 특정결합합니다.
    • WB, IP, IF와 ELISA으로 mouse, rat와 human origin의 ING4 and ING5을 검출할것을 권장합니다.
    • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ING4 Antibody (B-8) for WB applications. These reagents are now offered in bundles with ING4 Antibody (B-8) (see ordering information below).
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How much sample do you recommend loading on the gel for Western blot detection using ING4 (B-8): sc-515714?

Asked by: Trav11
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from ING4 (B8) scprovide a single immuno-band at MW 35K This antibody sc-515714 provide a single immuno-band with HepG2 cells. This is a replacement of the anti-ING4 (A8) sc376122 which gave multiple weak immuno-bands and did not give a supposed 29K immuno-band with total cells extract of many human cancer cell lines.
Date published: 2020-01-14
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