IL-8 Antibody (C-11): sc-376750

IL-8 Antibody (C-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects IL-8 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). IL-8 Antibody (C-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Interleukin-8, commonly referred to as IL-8, is a crucial member of the C-X-C chemokine family and serves as a potent chemoattractant cytokine that plays a vital role in the chemotaxis and activation of neutrophils, which are essential for the immune response. IL-8 is primarily secreted by activated macrophages and epithelial cells, and IL-8 expression is significantly associated with various chronic inflammatory diseases, such as inflammatory bowel disease and atherosclerosis. Notably, IL-8 is produced as a 99 amino acid precursor that is cleaved to yield a 72 amino acid, nonglycosylated, biologically active form. The dynamic equilibrium between IL-8 monomers and dimers, both in solution and bound to cell surfaces, is critical for regulating chemotaxis and receptor signaling. Research indicates that IL-8 dimerization acts as a negative regulator of IL-8 receptor function, with two receptors, IL-8RA and IL-8RB, exhibiting 77% sequence identity and functioning as seven-transmembrane domain proteins similar to G protein-coupled receptors. These receptors not only bind IL-8 but also interact with other members of the α and β chemokine families, underscoring IL-8′s importance in mediating inflammatory responses and potential as a therapeutic target in related diseases.

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IL-8 Antibody (C-11) References:

1. Dimer dissociation is essential for interleukin-8 (IL-8) binding to CXCR1 receptor.  |  Fernando, H., et al. 2004. J Biol Chem. 279: 36175-8. PMID: 15252057
2. SN38 increases IL-8 expression through the MAPK pathways in HCT8 cells.  |  Zhang, L., et al. 2017. Int J Mol Med. 39: 217-222. PMID: 27878250
3. CCAR2 negatively regulates IL-8 production in cervical cancer cells.  |  Kim, W., et al. 2018. Oncotarget. 9: 1143-1155. PMID: 29416683
4. Characterization of buffalo interleukin 8 (IL-8) and its expression in endometritis.  |  Abou Mossallam, AA., et al. 2015. J Genet Eng Biotechnol. 13: 71-77. PMID: 30647569
5. Plasma IL-8 and ICOSLG as prognostic biomarkers in glioblastoma.  |  Holst, CB., et al. 2021. Neurooncol Adv. 3: vdab072. PMID: 34286278
6. Neutrophil activation by monomeric interleukin-8.  |  Rajarathnam, K., et al. 1994. Science. 264: 90-2. PMID: 8140420
7. CXC chemokines bind to unique sets of selectivity determinants that can function independently and are broadly distributed on multiple domains of human interleukin-8 receptor B. Determinants of high affinity binding and receptor activation are distinct.  |  Ahuja, SK., et al. 1996. J Biol Chem. 271: 225-32. PMID: 8550564
8. Rapid mobilization of hematopoietic progenitor cells in rhesus monkeys by a single intravenous injection of interleukin-8.  |  Laterveer, L., et al. 1996. Blood. 87: 781-8. PMID: 8555503
9. Dansyl cadaverine regulates ligand induced endocytosis of interleukin-8 receptor in human polymorphonuclear neutrophils.  |  Ray, E. and Samanta, AK. 1996. FEBS Lett. 378: 235-9. PMID: 8557108
10. Selectivity and antagonism of chemokine receptors.  |  Wells, TN., et al. 1996. J Leukoc Biol. 59: 53-60. PMID: 8558067
11. Interleukin 8: cells of origin in inflammatory bowel disease.  |  Grimm, MC., et al. 1996. Gut. 38: 90-8. PMID: 8566866
12. Interleukin-8 regulation of the Ras/Raf/mitogen-activated protein kinase pathway in human neutrophils.  |  Knall, C., et al. 1996. J Biol Chem. 271: 2832-8. PMID: 8576262

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-IL-8 Antibody (C-11) has 22 citations in a variety of scientific publications.

Primary antibodies like Anti-IL-8 Antibody (C-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

IL-8 Antibody (C-11) Specifications

Alternate Product Name: Interleukin-8

Product Clone Name: C-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 71-99

Epitope Target: IL-8

Epitope Species: human

Product Reactivity/Detection: IL-8

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 22 citations in various scientific publications

Available Conjugates/Forms for IL-8 Antibody (C-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin