Date published: 2025-9-9

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IL-1 beta/IL1B 항체 (E7-2-hIL1β): sc-32294

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  • IL-1 beta/IL1B 항체(E7-2-hIL1β) 는 마우스 monoclonal IgG2bκ, 133간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • human origin의 IL-1β의 전체인 아미노산을 항원으로 사용하였습니다.
  • IL-1 beta/IL1B 항체 (E7-2-hIL1β)는 WB, IP, IF and IHC(P)으로 human유래의 IL-1β 를 감지하는 데에 추천한다 .
  • 항-IL-1 beta/IL1B 항체(E7-2-hIL1β)는 IP용 agarose와 결합되어 이용 가능하며, WB, IHC(P), ELISA용 HRP와 결합되어 이용 가능하며, IF, IHC(P), FCM용 phycoerythrin 또는 FITC와 결합되어 이용 가능합니다.
  • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • IL-1 beta/IL1B (E7-2-hIL1β): sc-32294무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgG Fc BP-HRPm-IgGκ BP-HRP 는IL-1 beta/IL1B 항체(E7-2-hIL1β) 가 WB and IHC(P) 에 응용될 때 우선으로 사용하는 이차 항체 검사 시약입니다.이 시약은IL-1 beta/IL1B 항체(E7-2-hIL1β) 와 세트로 제공드립니다. (아래의 주문 정보를 참고해 주시길 바랍니다)

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IL-1β 항체(E7-2-hIL1β)는 웨스턴 블롯(WB), 면역침전법(IP), 면역형광법(IF), 파라핀 포매 섹션(IHCP)을 이용한 면역조직화학법 등으로 인간 IL-1β를 검출하는 쥐 단일클론 IgG2b 카파 경쇄 항체입니다. IL-1β 단일 클론 항체(E7-2-hIL1β)는 비접합형과 아가로스, 과산화효소(HRP), 피코에리트린(PE), 플루오레세인 이소티오시아네이트(FITC), 다중 알렉사 플루오르® 접합체를 포함한 다양한 접합형 형태로 제공됩니다. IL-1β는 면역 반응과 염증을 매개하는 핵심 전염증성 사이토카인입니다. IL-1β는 주로 활성화된 대식세포에 의해 분비되며, T 및 B 림프구와 자연살해(NK) 세포를 활성화시킵니다. IL-1β는 세포질에 존재하며, 세포가 활성화되면 방출되어 IL-1 수용체에 결합하여 다른 염증 매개체를 생성하는 신호 전달 경로를 시작합니다. 이러한 세포질 위치 덕분에 염증 자극에 신속하게 반응할 수 있으므로, IL-1β는 신체 방어 메커니즘에 필수적입니다. IL-1β 생산의 조절 장애는 류마티스 관절염, 염증성 장 질환, 동맥 경화증 등 다양한 질환과 관련이 있으며, 건강과 질병에서 IL-1β의 중요성을 강조하고 있습니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

주문정보

제품명카탈로그 번호 단위가격수량관심품목

IL-1 beta/IL1B 항체 (E7-2-hIL1β)

sc-32294
200 µg/ml
$316.00

IL-1 beta/IL1B (E7-2-hIL1β): m-IgG Fc BP-HRP 번들

sc-528454
200 µg Ab; 10 µg BP
$354.00

IL-1 beta/IL1B (E7-2-hIL1β): m-IgGκ BP-HRP 번들

sc-520835
200 µg Ab, 40 µg BP
$354.00

IL-1 beta/IL1B 항체 (E7-2-hIL1β) AC

sc-32294 AC
500 µg/ml, 25% agarose
$416.00

IL-1 beta/IL1B 항체 (E7-2-hIL1β) HRP

sc-32294 HRP
200 µg/ml
$316.00

IL-1 beta/IL1B 항체 (E7-2-hIL1β) FITC

sc-32294 FITC
200 µg/ml
$330.00

IL-1 beta/IL1B 항체 (E7-2-hIL1β) PE

sc-32294 PE
200 µg/ml
$343.00

IL-1 beta/IL1B 항체 (E7-2-hIL1β) Alexa Fluor® 488

sc-32294 AF488
200 µg/ml
$357.00

IL-1 beta/IL1B 항체 (E7-2-hIL1β) Alexa Fluor® 546

sc-32294 AF546
200 µg/ml
$357.00

IL-1 beta/IL1B 항체 (E7-2-hIL1β) Alexa Fluor® 594

sc-32294 AF594
200 µg/ml
$357.00

IL-1 beta/IL1B 항체 (E7-2-hIL1β) Alexa Fluor® 647

sc-32294 AF647
200 µg/ml
$357.00

IL-1 beta/IL1B 항체 (E7-2-hIL1β) Alexa Fluor® 680

sc-32294 AF680
200 µg/ml
$357.00

IL-1 beta/IL1B 항체 (E7-2-hIL1β) Alexa Fluor® 790

sc-32294 AF790
200 µg/ml
$357.00

I would like to know if this IL-1B antibody ((E7-2-hIL1β): sc-32294) can detect pro-IL-1b and (mature) IL-1B. Thank you

Asked by: Anonymous
Thank you for your question. This antibody can detect both mature and precursor forms of IL1B in western blot.
Answered by: Tech Support Europe
Date published: 2025-08-04

这个抗体 “IL-1β 抗体 (E7-2-hIL1β): sc-32294”能用于检测猪的IL-1β吗?

Asked by: yingyingying
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

can I use this antibody(sc-32294) in mouse brain tissue for western blot?

Asked by: wcl6231
Thank you for your question. This antibody has not yet been tested in mouse and so that is not a recommended species at this time. It is human reactive only. We are still gathering data to show on our website. Check back for updates!
Answered by: Tech Service
Date published: 2020-01-15

Can IL-1β (E7-2-hILβ): sc-32294 monoclonal antibody be used in WB with zebrafish tissue ? Will there be non-specific staining?

Asked by: havana
Thank you for your question. This antibody has not been tested for use on zebrafish samples, so it is not recommended or warrantied for this use. However we do have a publication with this antibody in fish samples (PMID: 30589112). It is fine for WB applications.
Answered by: Tech Service
Date published: 2019-10-28

Can this il- 1b (sc 32294) detects mature form also..please clear

Asked by: Rahul89
Thank you for your question. Yes, this antibody, IL-1β Antibody (E7-2-hIL1β): sc-32294, is recommended for detection of precursor and mature IL-1beta. This has been confirmed through testing against the purified mature IL-1beta protein. In the cell lysates tested, we have also only observed the precursor form. We recommend you consult some of the product citations using this antibody to see if there is any recommendation for inducing expression of the mature form of the protein.
Answered by: Technical Support
Date published: 2019-07-12

Can IL-1β (E7-2-hILβ): sc-32294 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: DefinitelyNotMatt
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, IL-1β (E7-2-hILβ): sc-32294 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 2 out of 5 by from hl-60Because I used rabbit antibody had a good result, so I brought this again, but it didn't work in hl-60 cells.
Date published: 2020-09-19
Rated 1 out of 5 by from inconsistent for western blottingReceived a free sample of this antibody to test on AC16 cell lysates. Very high lot to lot variability. One lot showed only the mature band while the other showed only the precursor band for the same cell type. Antibody dilution used was 1:200.
Date published: 2018-04-27
Rated 2 out of 5 by from not work well for IF staining for rat brain tissueformaldehyde fixed rat brain tissue, get signal,but not good
Date published: 2017-10-24
Rated 5 out of 5 by from IHC stainingThe sample arrived soon and it worked well on the dilution 1:200 in IHC-P
Date published: 2017-10-20
Rated 4 out of 5 by from 此抗体功能很不错已经购买好几次这个抗体了,一如既往的好用。但是也有点缺点,就是有时候此蛋白的切割产物显示不出来,还是以前兔抗的更好用些。
Date published: 2017-09-21
Rated 5 out of 5 by from IL-1β使用评价大概是在八月份申请的试用装,进行WB试验还可以,效果挺不错的,符合试验预期的结果,希望贵公司越做越好
Date published: 2017-09-20
Rated 5 out of 5 by from IHCThe expression of IL-1 beta protein in the brain of rats with cerebral ischemia was analyzed by IHC,This antibody was very useful and we got expected staining which the signal is lower in treated group.1:800 dilution used.
Date published: 2017-09-16
Rated 5 out of 5 by from Very usefulVery useful antibody and it worked great.. Very little background and it is easily got the target band.I suggest use dilution 1:500 to detect this protein.
Date published: 2017-09-02
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IL-1 beta/IL1B 항체 (E7-2-hIL1β) is rated 4.1 out of 5 by 34.
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