IFRD1 항체 (D-7) WB, IP, IF와 ELISA으로 mouse, rat와 human origin의 IFRD1을 검출할것을 권장합니다.

IFRD1 항체 (D-7): sc-515012

IFRD1 항체 (D-7) is rated 3.3 out of 5 by 4.
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데이터 시트

    • 안티-IFRD1 항체 D-7 는 마우스 monoclonal IgG2b (kappa light chain) IFRD1 항체 이며 200 µg/ml으로 제공합니다.
    • human origin의 IFRD1에서 에 가까운 161-249 아미노산을 항원으로 사용하였습니다.
    • 안티-IFRD1 항체 (D-7)는 WB, IP, IF and ELISA으로 mouse, rat and human유래의 IFRD1 를 감지하는 데에 추천한다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-IFRD1 항체 (D-7)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • ChIP 에 사용하는 TransCruz시약(sc-515012 X, 200 µg/0.1 ml)이 있습니다.
    • IFRD1 (D-7): sc-515012무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for IFRD1 Antibody (D-7) for WB applications. This reagent is now offered in a bundle with IFRD1 Antibody (D-7) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with IFRD1 (D-7): sc-515012?

Asked by: jerojero
We recommend using one of our our exclusive Mouse IgG Binding Proteins conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support
Date published: 2017-02-27
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Rated 1 out of 5 by from not satisfactory A cytoplasmic staining is observed in the colon, as expected. However it could be a non-specific signal because it is related to single isolated cells rather than small clusters of cancer cells. The staining is totally non-specific in carotid plaques.
Date published: 2020-10-21
Rated 4 out of 5 by from Western blot It can use in western blot experiment, but two bands were obtained in our lab.
Date published: 2018-02-07
Rated 3 out of 5 by from 有杂带,按照说明书的对应位置曝光的不明显 很感谢能够申请到该抗体的小样使用。我们WB的是组织样本,按照本人所在实验室实验的wb条件进行实验,能够正常曝光,但发现曝光最先显出来的位置的分子量位置与说明书位置(53KDa)曝光位置不太一致,杂带比目标带曝光更明显,影响实验结果的判断。
Date published: 2018-01-31
Rated 5 out of 5 by from Good for mouse IFRD1 WB Started with dilution 1:200, got single, strong, clean band.
Date published: 2017-04-04
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