Date published: 2025-9-7

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GFP 항체 (1A5): sc-101536

4.5(8)
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데이터 시트
  • GFP 항체 1A5 는 랫드 monoclonal IgG1, 29간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • 재조합형에 대해 제기됨 GFP
  • WB, IP, IF와 ELISA으로 GFP and GFP fusion proteins 을 검출할것을 권장합니다.
  • 현재 GFP 항체(1A5)에 대해 선호하는 2차 검출 시약의 식별을 아직 완료하지 못했습니다. 이 작업은 진행 중입니다.

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녹색 형광 단백질(GFP)은 원래 해파리 Aequorea victoria의 생물 발광에 관여하는 단백질로 밝혀졌습니다. 외인성 기질이나 보조 인자 없이도 원핵세포에서 발현될 때 형광 생성물을 생성하는 GFP cDNA는 생체 내 유전자 발현과 단백질 위치를 모니터링하는 데 유용한 도구로 사용됩니다. 야생형 GFP보다 더 강렬하게 형광을 내는 EGFP를 비롯한 여러 GFP 돌연변이체가 개발되었습니다. 이러한 돌연변이체의 최대 여기치는 이중 표지 응용 분야뿐만 아니라 FACS 및 형광 현미경 검사에 더 유리합니다. GFP는 융합 단백질 태그로서 발현 벡터에 널리 사용되어 GFP에 융합된 이종 단백질의 발현 및 모니터링을 가능하게 합니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

주문정보

제품명카탈로그 번호 단위가격수량관심품목

GFP 항체 (1A5)

sc-101536
200 µg/ml
$316.00

Does this antibody(GFP Antibody (1A5)) works for venus? If not, do you have any idea which GFP antibody here can recognize Venus?

Asked by: Liu M
Thank you for your question. Please contact our Technical Service Department with the specific protein sequence for Venus, and we can perform sequence analysis of our GFP antibody antigens to see if any of them may also detect this protein. We do not have any data or citations for this GFP antibody specifically mentioning reactivity with Venus.
Answered by: Technical Support
Date published: 2020-03-03

Could you tell me which regions or amino acid of the GFP protein is targeted by this antibody?

Asked by: jfoul78
Thank you for your question. GFP Antibody (1A5) is a rat monoclonal raised against recombinant GFP. Unfortunately, the exact sequence used as an immunogen is not known, nor is the epitope recognized by this specific clone. We do offer a broad variety of GFP antibodies where this information is available. For example, our GFP Antibody (C-2): sc-390394 is specific for an epitope mapping between amino acids 167-182 within an internal region of GFP of Aequorea victoria origin. Please contact our Technical Service Department for help in choosing the best antibody for your research needs. Please call us toll-free at (800) 457-3801 option 2, email scbt@scbt.com, or use our live chat.
Answered by: Technical Support
Date published: 2017-02-03

I would like to use GFP Antibody (1A5): sc-101536 for double staining with another mouse monoclonal primary antibody. Is this possible?

Asked by: Trav11
Thank you for your question. In cases like this, we recommend using our directly conjugated mouse monoclonal primary antibodies. Currently, sc-101536 is not available directly conjugated, but we have an alternative GFP antibody (sc-9996) that is available conjugated to HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Technical Support 7
Date published: 2017-01-24

I am looking for a GFP antibody that was raised against the amino acid sequence 215-230 (RDHMVL LEFVTAAGIT). Do you have any antibody like this? Thanks!

Asked by: Anette
Thank you for your question. Most of our GFP antibodies were raised against full length recombinant GFP, which includes this portion of the sequence that you have specified. However, the exact epitope recognized by each monoclonal antibody has not been determined. If you would be interested in testing a complimentary sample size of our mouse monoclonal antibody, GFP (B-2): sc-9996, to see if it would work for your purposes, please contact our Technical Service Department. Please call us directly at (800) 457-3801 option 2, email scbt@scbt.com, or use our live chat.
Answered by: Technical Support
Date published: 2017-01-19
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Rated 1 out of 5 by from Did not work in texted tissue samplesI got a free sample and tested it according to th eworking lab protocol.There was no signal in the blot.
Date published: 2018-04-09
Rated 5 out of 5 by from Positive WB and IFAntibody is positive for both westren blot and IF staining
Date published: 2017-02-05
Rated 5 out of 5 by from Reliable antibodyGood choice if you're looking for a reliable GFP antibody to use in WB and IF.
Date published: 2017-01-11
Rated 5 out of 5 by from Strong bands at 1:1000 dilutionStrong bands at 1:1000 dilution.
Date published: 2015-09-10
Rated 5 out of 5 by from Worked well in WBWorked well in WB, no complaints.
Date published: 2015-05-27
Rated 5 out of 5 by from Good antibody for western blot analysis ofGood antibody for western blot analysis of GFP expression in 293T cell lysate. -SCBT QC
Date published: 2015-04-07
Rated 5 out of 5 by from Work well for IF staining of COS cellsWork well for IF staining of COS cells. -SCBT QC
Date published: 2015-02-01
Rated 5 out of 5 by from Generated publishable data on Hela cellsGenerated publishable data on Hela cells with this antibody. -SCBT Publication Review
Date published: 2013-11-23
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GFP 항체 (1A5) is rated 4.5 out of 5 by 8.
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