Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
G9a Double Nickase Plasmid (h): sc-402484-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- G9a Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- G9a Double Nickase Plasmid (h) and G9a Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting EHMT2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: G9a Antibody (C-9): sc-515726
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
G9a Double Nickase Plasmid (h) | sc-402484-NIC | 20 µg | $410.00 | |||
G9a Double Nickase Plasmid (h2) | sc-402484-NIC-2 | 20 µg | $410.00 |
EHMT2 encodes the lysine methyltransferase G9a, a principal writer of H3K9me1/2 that establishes repressive chromatin and supports stable gene silencing across development and lineage specification. G9a functions in nuclear epigenetic complexes to coordinate chromatin organization, transcriptional programs, DNA damage responses, and crosstalk with other histone and DNA methylation pathways. Altered EHMT2/G9a activity has been linked to widespread transcriptional deregulation observed in cancer biology, neurodevelopmental phenotypes, and inflammatory signaling, making it a common node in studies of epigenetic plasticity. Investigating G9a-dependent H3K9 methylation helps define how chromatin state influences proliferation, differentiation, and stress-adaptive transcriptional networks in human cells.
G9a Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the EHMT2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within EHMT2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt EHMT2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of EHMT2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.