G9a Antibody (C-9): sc-515726

G9a Antibody (C-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects G9a protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-G9a antibody (C-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. G9a protein, also known as EHMT2, plays a crucial role in the regulation of gene expression through its function as a histone methyltransferase, specifically targeting histone H3 lysine 9 for methylation. This post-translational modification is vital for the repression of developmental genes and is essential for early embryogenesis, highlighting G9a′s importance in cellular differentiation and development. G9a′s ability to add methyl groups to lysine residues not only influences transcriptional regulation but also contributes to the organization of chromatin structure, thereby impacting overall genomic stability. Human G9a gene is located on chromosome 6p21.3, and expression is tightly regulated, reflecting significance in various biological processes. With high activity compared to other methyltransferases, G9a is a key player in the epigenetic landscape, making G9a (C-9) monoclonal antibody an invaluable tool for researchers studying gene regulation and chromatin dynamics.

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G9a Antibody (C-9) References:

1. Set domain-containing protein, G9a, is a novel lysine-preferring mammalian histone methyltransferase with hyperactivity and specific selectivity to lysines 9 and 27 of histone H3.  |  Tachibana, M., et al. 2001. J Biol Chem. 276: 25309-17. PMID: 11316813
2. Novel NG36/G9a gene products encoded within the human and mouse MHC class III regions.  |  Brown, SE., et al. 2001. Mamm Genome. 12: 916-24. PMID: 11707778
3. G9a histone methyltransferase plays a dominant role in euchromatic histone H3 lysine 9 methylation and is essential for early embryogenesis.  |  Tachibana, M., et al. 2002. Genes Dev. 16: 1779-91. PMID: 12130538
4. Human major histocompatibility complex contains a minimum of 19 genes between the complement cluster and HLA-B.  |  Spies, T., et al. 1989. Proc Natl Acad Sci U S A. 86: 8955-8. PMID: 2813433
5. Histone methyltransferase G9a protects against acute liver injury through GSTP1.  |  Zhang, Y., et al. 2020. Cell Death Differ. 27: 1243-1258. PMID: 31515511
6. G9a Modulates Lipid Metabolism in CD4 T Cells to Regulate Intestinal Inflammation.  |  Ramos, GP., et al. 2023. Gastroenterology. 164: 256-271.e10. PMID: 36272457
7. PALI1 promotes tumor growth through competitive recruitment of PRC2 to G9A-target chromatin for dual epigenetic silencing.  |  Fong, KW., et al. 2022. Mol Cell. 82: 4611-4626.e7. PMID: 36476474
8. G9a promotes immune suppression by targeting the Fbxw7/Notch pathway in glioma stem cells.  |  Cao, Y., et al. 2023. CNS Neurosci Ther. 29: 2508-2521. PMID: 36971192
9. Depletion of G9A attenuates imiquimod-induced psoriatic dermatitis via targeting EDAR-NF-κB signaling in keratinocyte.  |  Fang, Z., et al. 2023. Cell Death Dis. 14: 627. PMID: 37739945
10. The dopamine transporter antagonist vanoxerine inhibits G9a and suppresses cancer stem cell functions in colon tumors.  |  Bergin, CJ., et al. 2024. Nat Cancer. 5: 463-480. PMID: 38351181
11. The bach1/G9a/Slc7a11 axis epigenetically promotes renal fibrosis by mediated ferroptosis.  |  Yao, X., et al. 2024. Int Immunopharmacol. 143: 113363. PMID: 39393269
12. The G9a gene in the human major histocompatibility complex encodes a novel protein containing ankyrin-like repeats.  |  Milner, CM. and Campbell, RD. 1993. Biochem J. 290 (Pt 3): 811-8. PMID: 8457211

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-G9a Antibody (C-9) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-G9a Antibody (C-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

G9a Antibody (C-9) Specifications

Alternate Product Name: EHMT2

Product Clone Name: C-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 346-564

Epitope Target: G9a

Epitope Species: human

Product Reactivity/Detection: G9a

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for G9a Antibody (C-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin