Flg 항체 (M2F12) WB, IP, IF와 IHC(P)으로 mouse, rat와 human origin의 Flg isoform α을 검출할것을 권장합니다.

Flg 항체 (M2F12): sc-57132

Flg 항체 (M2F12) is rated 4.5 out of 5 by 11.
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Datasheets

    • 안티-Flg 항체 M2F12 는 마우스 monoclonal IgG2a (kappa light chain) Flg 항체 이며 200 µg/ml으로 제공합니다.
    • raised against the ectodomain of Flg isoform α of human origin
    • 안티-Flg 항체 (M2F12)는 WB, IP, IF and IHC(P)으로 mouse, rat and human유래의 Flg isoform α 를 감지하는 데에 추천한다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-Flg 항체 (M2F12)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • 강력추천하는 conventional secondary anti-mouse IgG reagents의 대체제품 보기m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP).
    • Flg (M2F12): sc-57132무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
이 제품의 리뷰를 쓰고 15 CruzCredits를 받기

Can this antibody detect FGFR1 isoform beta? I'm trying to blot for endogenous FGFR1 in PC3 cell line, and I'm not getting a signal so I wonder if PC3 cells are expressing only beta form and the antibody cannot detect or something.

Asked by: Juko
Thank you very much for your question. This antibody was raised against Flg isoform α (ectodomain) of human origin. Unfortunately, we have not tested this antibody on PC3 cells. Our labs suggest as positive controls e.g. PC-12, HeLa, SK-N-SH, Neuro-2A and U-87 MG whole cell lysates (we do provide all these in case you are interested). Please contact our Technical Service Department by phone, e-mail or live chat in case you feel the WB results could be improved by changing the protocol settings. We are happy to help you troubleshoot the antibody. Santa Cruz Biotechnology does warranty our antibodies for one year from the date of purchase. If we are unable to successfully troubleshoot your application and get a signal at the expected mw size in a positive sample material, we will send you a replacement antibody free of charge.
Answered by: Technical Support Europe
Date published: 2017-09-18

Can Flg (M2F12): sc-57132 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: jerojero
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Flg (M2F12): sc-57132 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-09-15
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Rated 5 out of 5 by from work well in western blot at 1:200,ov90 cell Although it looked like just so so.But it actually worked very well, so it is good
Date published: 2017-09-20
Rated 5 out of 5 by from Immunofluorescence I used it (Flg (M2F12)) for immunofluorescence at LX-2 cell (dilution 1:200), and appeared blight image.
Date published: 2017-07-05
Rated 5 out of 5 by from Great antibody The antibody is excellent in Western Blotting and IHC
Date published: 2017-03-22
Rated 5 out of 5 by from Strong antibody for IHC and WB Nice specific staining on paraffin sections, very clean and strong results. It's also a great choice for Western blot! Smart choice!
Date published: 2017-02-01
Rated 5 out of 5 by from Pulito e molto specifico Ho ricevuto 10 µg di anticorpo in prova e sono riuscito a vedere un segnale di FGFR1 finalmente pulito, chiaro ed inconfutabile. Mi ha risolto un problema che andava avanti da mesi.
Date published: 2017-01-02
Rated 4 out of 5 by from Positive band detected in WB in K Positive band detected in WB in K-562 whole cell lysate. -SCBT Publication Review
Date published: 2015-05-10
Rated 4 out of 5 by from Great immunofluorescence cytoplasmic staining Great immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-04-06
Rated 4 out of 5 by from Good band detected in WB in HeLa whole cell lysate Good band detected in WB in HeLa whole cell lysate. -SCBT QC
Date published: 2015-02-22
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