Anticorps Flg (M2F12) recommandé pour la détection de Flg isoform α d'origine mouse, rat et human par WB, IP, IF et IHC(P)

Anticorps Flg (M2F12): sc-57132

Anticorps Flg (M2F12) est évalué 4.5 de 5 de 11.
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Fiches techniques

    • Anti-L'anticorps Flg M2F12 est un monoclonal IgG2a (chaîne légère kappa) L'Anticorps Flg fourni en 200 µg/ml
    • raised against the ectodomain of Flg isoform α of human origin
    • Anti-L'Anticorps Flg (M2F12) est recommandé pour la détection de Flg isoform α d'origine mouse, rat et human par WB, IP, IF et IHC(P)
    • Anti-L'Anticorps Flg (M2F12) est disponible conjugué à l'agarose pour IP; à l'HRP pour WB, IHC(P) et ELISA; et soit à la phycoerythrin ou FITC pour IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 pour WB (RGB), IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 680 ou Alexa Fluor® 790 pour WB (NIR), IF et FCM
    • Voir m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), nos recombinants vivement recommandés en alternative des réactifs IgG secondaires anti-souris conventionels.
    • Contactez notre Service Technique (ou votre Distributeur local) pour plus d'informations pour recevoir un échantillon GRATUIT de 10 µg de Flg (M2F12): sc-57132.
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Can this antibody detect FGFR1 isoform beta? I'm trying to blot for endogenous FGFR1 in PC3 cell line, and I'm not getting a signal so I wonder if PC3 cells are expressing only beta form and the antibody cannot detect or something.

Posée par: Juko
Thank you very much for your question. This antibody was raised against Flg isoform α (ectodomain) of human origin. Unfortunately, we have not tested this antibody on PC3 cells. Our labs suggest as positive controls e.g. PC-12, HeLa, SK-N-SH, Neuro-2A and U-87 MG whole cell lysates (we do provide all these in case you are interested). Please contact our Technical Service Department by phone, e-mail or live chat in case you feel the WB results could be improved by changing the protocol settings. We are happy to help you troubleshoot the antibody. Santa Cruz Biotechnology does warranty our antibodies for one year from the date of purchase. If we are unable to successfully troubleshoot your application and get a signal at the expected mw size in a positive sample material, we will send you a replacement antibody free of charge.
Répondue par: Technical Support Europe
Date de publication: 2017-09-18

Can Flg (M2F12): sc-57132 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Posée par: jerojero
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Flg (M2F12): sc-57132 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Répondue par: Technical Support
Date de publication: 2017-09-15
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Rated 5 de 5 de par work well in western blot at 1:200,ov90 cell Although it looked like just so so.But it actually worked very well, so it is good
Date de publication: 2017-09-20
Rated 5 de 5 de par Immunofluorescence I used it (Flg (M2F12)) for immunofluorescence at LX-2 cell (dilution 1:200), and appeared blight image.
Date de publication: 2017-07-05
Rated 5 de 5 de par Great antibody The antibody is excellent in Western Blotting and IHC
Date de publication: 2017-03-22
Rated 5 de 5 de par Strong antibody for IHC and WB Nice specific staining on paraffin sections, very clean and strong results. It's also a great choice for Western blot! Smart choice!
Date de publication: 2017-02-01
Rated 5 de 5 de par Pulito e molto specifico Ho ricevuto 10 µg di anticorpo in prova e sono riuscito a vedere un segnale di FGFR1 finalmente pulito, chiaro ed inconfutabile. Mi ha risolto un problema che andava avanti da mesi.
Date de publication: 2017-01-02
Rated 4 de 5 de par Positive band detected in WB in K Positive band detected in WB in K-562 whole cell lysate. -SCBT Publication Review
Date de publication: 2015-05-10
Rated 4 de 5 de par Great immunofluorescence cytoplasmic staining Great immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date de publication: 2015-04-06
Rated 4 de 5 de par Good band detected in WB in HeLa whole cell lysate Good band detected in WB in HeLa whole cell lysate. -SCBT QC
Date de publication: 2015-02-22
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