EF-Ts Antibody (A-10): sc-271588
- EF-Ts Antibody (A-10) is a mouse monoclonal IgG1 κ EF-Ts antibody provided at 200 µg/ml
- raised against amino acids 36-325 mapping at the C-terminus of EF-Ts of human origin
- EF-Ts Antibody (A-10) is recommended for detection of EF-Ts of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-EF-Ts Antibody (A-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for EF-Ts Antibody (A-10) for WB applications. These reagents are now offered in bundles with EF-Ts Antibody (A-10) (see ordering information below).
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EF-Ts Antibody (A-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects EF-Ts protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). EF-Ts Antibody (A-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. EF-Ts (TSFM) is a crucial 325 amino acid protein that plays a vital role in the elongation phase of protein biosynthesis on the ribosome, being one of the 13 mitochondrial-encoded proteins involved in this process. EF-Ts (TSFM) is predominantly expressed in the liver, kidney, and skeletal muscle, where EF-Ts (TSFM) interacts with EF-Tu, a multidomain GTPase essential for translation. This interaction facilitates the exchange of guanosine diphosphate for guanosine triphosphate, thereby promoting protein elongation. Mutations in EF-Ts (TSFM) can lead to combined oxidative phosphorylation deficiency type 3 (COXPD3), a serious condition marked by defects in the mitochondrial oxidative phosphorylation system, often resulting in severe metabolic acidosis and complications such as encephalomyopathy or hypertrophic cardiomyopathy. Multiple isoforms of EF-Ts (TSFM) arise from alternative splicing events, which may have implications for functional diversity and regulation in various cellular contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
EF-Ts Antibody (A-10) References:
- Assignment of the mitochondrial translation elongation factor Ts gene (TSFM) to human chromosome 12 bands q13-->q14 by in situ hybridization and with somatic cell hybrids. | Vernon, JL., et al. 2000. Cytogenet Cell Genet. 89: 145-6. PMID: 10965106
- The molecular basis for tissue specificity of the oxidative phosphorylation deficiencies in patients with mutations in the mitochondrial translation factor EFG1. | Antonicka, H., et al. 2006. Hum Mol Genet. 15: 1835-46. PMID: 16632485
- Distinct clinical phenotypes associated with a mutation in the mitochondrial translation elongation factor EFTs. | Smeitink, JA., et al. 2006. Am J Hum Genet. 79: 869-77. PMID: 17033963
- Cloning and expression of mitochondrial translational elongation factor Ts from bovine and human liver. | Xin, H., et al. 1995. J Biol Chem. 270: 17243-9. PMID: 7615523
- Interaction of mitochondrial elongation factors Tu.Ts with aminoacyl-tRNA. | Benkowski, LA., et al. 1995. Nucleic Acids Symp Ser. 163-6. PMID: 8643359
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EF-Ts Antibody (A-10) | sc-271588 | 200 µg/ml | $322.00 | |||
EF-Ts Antibody (A-10): m-IgG Fc BP-HRP Bundle | sc-529216 | 200 µg Ab; 10 µg BP | $361.00 | |||
EF-Ts Antibody (A-10): m-IgGκ BP-HRP Bundle | sc-521989 | 200 µg Ab, 40 µg BP | $361.00 | |||
EF-Ts Antibody (A-10): m-IgG1 BP-HRP Bundle | sc-543393 | 200 µg Ab; 20 µg BP | $361.00 | |||
EF-Ts Antibody (A-10) AC | sc-271588 AC | 500 µg/ml, 25% agarose | $424.00 | |||
EF-Ts Antibody (A-10) HRP | sc-271588 HRP | 200 µg/ml | $322.00 | |||
EF-Ts Antibody (A-10) FITC | sc-271588 FITC | 200 µg/ml | $336.00 | |||
EF-Ts Antibody (A-10) PE | sc-271588 PE | 200 µg/ml | $349.00 | |||
EF-Ts Antibody (A-10) Alexa Fluor® 488 | sc-271588 AF488 | 200 µg/ml | $364.00 | |||
EF-Ts Antibody (A-10) Alexa Fluor® 546 | sc-271588 AF546 | 200 µg/ml | $364.00 | |||
EF-Ts Antibody (A-10) Alexa Fluor® 594 | sc-271588 AF594 | 200 µg/ml | $364.00 | |||
EF-Ts Antibody (A-10) Alexa Fluor® 647 | sc-271588 AF647 | 200 µg/ml | $364.00 | |||
EF-Ts Antibody (A-10) Alexa Fluor® 680 | sc-271588 AF680 | 200 µg/ml | $364.00 | |||
EF-Ts Antibody (A-10) Alexa Fluor® 790 | sc-271588 AF790 | 200 µg/ml | $364.00 |