DNA pol α Antibody (F-6): sc-365040
- DNA pol α Antibody (F-6) is a mouse monoclonal IgG2b κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 3-31 near the N-terminus of DNA pol α of human origin
- recommended for detection of DNA pol α of mouse, rat and human origin by WB, IP, IF and ELISA
- m-IgG2b BP-HRP is the preferred secondary detection reagent for DNA pol α Antibody (F-6) for WB applications. This reagent is now offered in a bundle with DNA pol α Antibody (F-6) (see ordering information below).
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DNA pol α Antibody (F-6) is a mouse monoclonal IgG2b antibody that detects DNA polymerase alpha (DNA pol α) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DNA pol α plays a crucial role in DNA replication, particularly in the synthesis of the RNA-DNA primer necessary for initiating DNA strand elongation. DNA pol α is essential for maintaining genomic stability, ensuring accurate DNA replication and repair processes. DNA pol α belongs to a larger family of DNA polymerases, including DNA pol δ and DNA pol ε, which work together to manage DNA synthesis and repair. DNA pol α function remains vital, as deficiencies or malfunctions can lead to genomic instability and contribute to various diseases, including cancer. Anti-DNA pol α antibody (F-6) enables researchers to study expression and activity in different cellular contexts, providing insights into DNA replication mechanisms and potential therapeutic interventions.
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DNA pol α Antibody (F-6) References:
- Variations of DNA polymerase-alpha and -beta during prolonged stimulation of human lymphocytes. | Bertazzoni, U., et al. 1976. Proc Natl Acad Sci U S A. 73: 785-9. PMID: 1062788
- Stimulation of DNA polymerase alpha by hypergravity generated by centrifugal acceleration. | Takemura, M. and Yoshida, S. 2001. Biochem Biophys Res Commun. 289: 345-9. PMID: 11716478
- Inhibition of DNA excision by DNA polymerase-alpha inhibitor in UV-damaged HeLa cells. | Joe, CO., et al. 1990. Toxicol Lett. 52: 253-9. PMID: 2117783
- Role of swi7H4 mutant allele of DNA polymerase α in the DNA damage checkpoint response. | Khan, S. and Ahmed, S. 2015. PLoS One. 10: e0124063. PMID: 25822347
- The antitumor toxin CD437 is a direct inhibitor of DNA polymerase α. | Han, T., et al. 2016. Nat Chem Biol. 12: 511-5. PMID: 27182663
- DNA polymerase alpha activity is not affected by protein kinases or alkaline phosphatase. | Prussak, CE. and Tseng, BY. 1989. Biochem Biophys Res Commun. 159: 1397-403. PMID: 2930569
- Lability of DNA polymerase alpha correlated with decreased DNA synthesis and increased age in human cells. | Busbee, D., et al. 1987. J Natl Cancer Inst. 79: 1231-9. PMID: 3480375
- Monoclonal antibodies against human DNA polymerase-alpha inhibit DNA replication in permeabilized human cells. | Miller, MR., et al. 1985. J Biol Chem. 260: 134-8. PMID: 3917431
- Enzymological characterization of DNA polymerase alpha. Basic catalytic properties processivity, and gap utilization of the homogeneous enzyme from human KB cells. | Fisher, PA., et al. 1979. J Biol Chem. 254: 6128-37. PMID: 447699
- Mitochondrial DNA replication does not involve DNA polymerase alpha. | Zimmermann, W., et al. 1980. J Biol Chem. 255: 11847-52. PMID: 6777385
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DNA pol α Antibody (F-6) | sc-365040 | 200 µg/ml | $322.00 | |||
DNA pol α Antibody (F-6): m-IgG2b BP-HRP Bundle | sc-548548 | 200 µg Ab; 10 µg BP | $361.00 | |||
DNA pol α (F-6) Neutralizing Peptide | sc-365040 P | 100 µg/0.5 ml | $69.00 |