DACH1 Antibody (A-6): sc-398706
- DACH1 Antibody (A-6) is a mouse monoclonal IgG2a κ DACH1 antibody provided at 200 µg/ml
- raised against amino acids 528-620 mapping within an internal region of DACH1 of human origin
- DACH1 Antibody (A-6) is recommended for detection of DACH1 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and bovine and porcine
- Anti-DACH1 Antibody (A-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-398706 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for DACH1 Antibody (A-6) for WB applications. These reagents are now offered in bundles with DACH1 Antibody (A-6) (see ordering information below).
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DACH1 Antibody (A-6) is a mouse monoclonal IgG2a kappa light chain antibody that detects DACH1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DACH1 Antibody (A-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DACH1, or Dachshund homolog 1, is a 758 amino acid protein that serves as a crucial transcription factor localized in the nucleus, playing a significant role in the regulation of gene expression during organogenesis, particularly in eye development. DACH1 inhibits the TGF-beta signaling pathway through interactions with SMAD4 and NCOR1, which is vital for maintaining proper cellular functions and preventing aberrant growth. Additionally, DACH1 has been implicated in tumor suppression, as DACH1 can block cellular proliferation and inhibit the growth of human breast cancer cells. Notably, DACH1 exists in four isoforms due to alternative splicing, which may contribute to diverse regulatory functions in various tissues.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
DACH1 Antibody (A-6) References:
- DACH: genomic characterization, evaluation as a candidate for postaxial polydactyly type A2, and developmental expression pattern of the mouse homologue. | Ayres, JA., et al. 2001. Genomics. 77: 18-26. PMID: 11543628
- Dach1, a vertebrate homologue of Drosophila dachshund, is expressed in the developing eye and ear of both chick and mouse and is regulated independently of Pax and Eya genes. | Heanue, TA., et al. 2002. Mech Dev. 111: 75-87. PMID: 11804780
- DACH1 inhibits transforming growth factor-beta signaling through binding Smad4. | Wu, K., et al. 2003. J Biol Chem. 278: 51673-84. PMID: 14525983
- Expression profiling identifies altered expression of genes that contribute to the inhibition of transforming growth factor-beta signaling in ovarian cancer. | Sunde, JS., et al. 2006. Cancer Res. 66: 8404-12. PMID: 16951150
- DACH1 is a cell fate determination factor that inhibits cyclin D1 and breast tumor growth. | Wu, K., et al. 2006. Mol Cell Biol. 26: 7116-29. PMID: 16980615
- Cell fate determination factor DACH1 inhibits c-Jun-induced contact-independent growth. | Wu, K., et al. 2007. Mol Biol Cell. 18: 755-67. PMID: 17182846
- Progenitor cells from the porcine neural retina express photoreceptor markers after transplantation to the subretinal space of allorecipients. | Klassen, H., et al. 2007. Stem Cells. 25: 1222-30. PMID: 17218397
- DACH1 negatively regulates the human RANK ligand gene expression in stromal/preosteoblast cells. | Sundaram, K., et al. 2008. J Cell Biochem. 103: 1747-59. PMID: 17891780
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DACH1 Antibody (A-6) | sc-398706 | 200 µg/ml | $322.00 | |||
DACH1 Antibody (A-6): m-IgG Fc BP-HRP Bundle | sc-526320 | 200 µg Ab; 10 µg BP | $361.00 | |||
DACH1 Antibody (A-6): m-IgG2a BP-HRP Bundle | sc-546582 | 200 µg Ab; 10 µg BP | $361.00 | |||
DACH1 Antibody (A-6) X | sc-398706 X | 200 µg/0.1 ml | $322.00 | |||
DACH1 Antibody (A-6) AC | sc-398706 AC | 500 µg/ml, 25% agarose | $424.00 | |||
DACH1 Antibody (A-6) HRP | sc-398706 HRP | 200 µg/ml | $322.00 | |||
DACH1 Antibody (A-6) FITC | sc-398706 FITC | 200 µg/ml | $336.00 | |||
DACH1 Antibody (A-6) PE | sc-398706 PE | 200 µg/ml | $349.00 | |||
DACH1 Antibody (A-6) Alexa Fluor® 488 | sc-398706 AF488 | 200 µg/ml | $364.00 | |||
DACH1 Antibody (A-6) Alexa Fluor® 546 | sc-398706 AF546 | 200 µg/ml | $364.00 | |||
DACH1 Antibody (A-6) Alexa Fluor® 594 | sc-398706 AF594 | 200 µg/ml | $364.00 | |||
DACH1 Antibody (A-6) Alexa Fluor® 647 | sc-398706 AF647 | 200 µg/ml | $364.00 | |||
DACH1 Antibody (A-6) Alexa Fluor® 680 | sc-398706 AF680 | 200 µg/ml | $364.00 | |||
DACH1 Antibody (A-6) Alexa Fluor® 790 | sc-398706 AF790 | 200 µg/ml | $364.00 |