안티-cystatin C 항체 A-10 는 마우스 monoclonal IgG1 (kappa light chain) cystatin C 항체 이며 200 µg/ml으로 제공합니다.
아미노 산 26-87 mapping within an internal region of cystatin C of human 의하고 기원한다
cystatin C 항체 (A-10)는 WB, IP, IF and ELISA으로 human유래의 cystatin C 를 감지하는 데에 추천한다 .
IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-cystatin C 항체 (A-10)를 제공한다.
WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for cystatin C Antibody (A-10) for WB applications. This reagent is now offered in a bundle with cystatin C Antibody (A-10) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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cystatin C Antibody (A-10) is a high quality monoclonal cystatin C antibody (also designated CST3 antibody) suitable for the detection of the cystatin C protein of human origin. cystatin C Antibody (A-10) is available as both the non-conjugated anti-cystatin C antibody form, as well as multiple conjugated forms of anti-cystatin C antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Cystatin C is a cysteine (thiol) protease inhibitor that belongs to the type II cystatin gene superfamily and is the most abundant extracellular inhibitor of cysteine proteases. Cystatin C is a constitutively secreted, amyloidogenic protein, which forms a two-fold symmetric dimer and modulates both cysteine protease activity and the expression of class II MHC molecules. Expression of cystatin C is an indicator of kidney function and glomerular filtration rate. Mutations in the cystatin C gene can lead to protein aggregates, which are implicated in hereditary amyloid angiopathy (HCCAA) and cerebral hemorrhage. Although both wild-type and mutant cystatin C are capable of forming concentration dependent inactive dimers, mutant cystatin C dimerizes at lower concentrations and is more susceptible to serine proteases, which may facilitate aggregation. In neuronal cells, oxidative stress stimulates expression of cystatin C, which may positively regulate apoptosis.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
How much sample do you recommend loading on the gel for Western blot detection using cystatin C (A-10): sc-515732?
Asked by: DefinitelyNotMatt
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: