cyclin B2 Antibody (A-2): sc-28303
- cyclin B2 Antibody (A-2) is a mouse monoclonal IgG1 κ cyclin B2 antibody, cited in 16 publications, provided at 200 µg/ml
- raised against amino acids 1-105 of cyclin B2 of human origin
- cyclin B2 Antibody (A-2) is recommended for detection of cyclin B2 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-cyclin B2 Antibody (A-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for cyclin B2 Antibody (A-2) for WB applications. These reagents are now offered in bundles with cyclin B2 Antibody (A-2) (see ordering information below).
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cyclin B2 Antibody (A-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects cyclin B2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-cyclin B2 antibody (A-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. cyclin B2 plays a crucial role in regulating cell cycle, particularly during transition from G2 phase to mitosis. cyclin B2 functions by forming a complex with cyclin-dependent kinase Cdc2, which is essential for activating maturation-promoting factor (MPF). This activation is vital for proper progression of mitosis, triggering phosphorylation of various substrates that lead to chromatin condensation, nuclear envelope breakdown, and spindle formation. Precise regulation of cyclin B2 and Cdc2 interaction is critical, as dysregulation can lead to uncontrolled cell division and is often implicated in cancer. cyclin B2 monoclonal antibody (A-2) serves as a valuable tool for researchers studying cell cycle dynamics and molecular mechanisms underlying mitotic regulation.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
cyclin B2 Antibody (A-2) References:
- Cyclin B in Xenopus oocytes: implications for the mechanism of pre-MPF activation. | Gautier, J. and Maller, JL. 1991. EMBO J. 10: 177-82. PMID: 1824935
- Specific activation of cdc25 tyrosine phosphatases by B-type cyclins: evidence for multiple roles of mitotic cyclins. | Galaktionov, K. and Beach, D. 1991. Cell. 67: 1181-94. PMID: 1836978
- Cyclin is a component of maturation-promoting factor from Xenopus. | Gautier, J., et al. 1990. Cell. 60: 487-94. PMID: 1967981
- Control of M-phase by maturation-promoting factor. | Dorée, M. 1990. Curr Opin Cell Biol. 2: 269-73. PMID: 2141993
- Direct activation of cdc2 with phosphatase: identification of p13suc1-sensitive and insensitive steps. | Jessus, C., et al. 1990. FEBS Lett. 266: 4-8. PMID: 2163887
- Reversible tyrosine phosphorylation of cdc2: dephosphorylation accompanies activation during entry into mitosis. | Morla, AO., et al. 1989. Cell. 58: 193-203. PMID: 2473839
- Dominoes and clocks: the union of two views of the cell cycle. | Murray, AW. and Kirschner, MW. 1989. Science. 246: 614-21. PMID: 2683077
- The cyclin B2/CDK1 complex inhibits separase activity in mouse oocyte meiosis I. | Li, J., et al. 2019. Development. 146: PMID: 31704793
- The Cyclin B2/CDK1 Complex Conservatively Inhibits Separase Activity in Oocyte Meiosis II. | Li, J., et al. 2021. Front Cell Dev Biol. 9: 648053. PMID: 33777955
- Mad2 promotes Cyclin B2 recruitment to the kinetochore for guiding accurate mitotic checkpoint. | Liu, S., et al. 2022. EMBO Rep. 23: e54171. PMID: 35384228
- Extracellular vesicle-derived CircWhsc1 promotes cardiomyocyte proliferation and heart repair by activating TRIM59/STAT3/Cyclin B2 pathway. | Wei, G., et al. 2022. J Adv Res.. PMID: 36587763
- Isolation of the murine cyclin B2 cDNA and characterization of the lineage and temporal specificity of expression of the B1 and B2 cyclins during oogenesis, spermatogenesis and early embryogenesis. | Chapman, DL. and Wolgemuth, DJ. 1993. Development. 118: 229-40. PMID: 8375336
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
cyclin B2 Antibody (A-2) | sc-28303 | 200 µg/ml | $322.00 | |||
cyclin B2 Antibody (A-2): m-IgG Fc BP-HRP Bundle | sc-526658 | 200 µg Ab; 10 µg BP | $361.00 | |||
cyclin B2 Antibody (A-2): m-IgG1 BP-HRP Bundle | sc-532031 | 200 µg Ab; 20 µg BP | $361.00 | |||
cyclin B2 Antibody (A-2) AC | sc-28303 AC | 500 µg/ml, 25% agarose | $424.00 | |||
cyclin B2 Antibody (A-2) HRP | sc-28303 HRP | 200 µg/ml | $322.00 | |||
cyclin B2 Antibody (A-2) FITC | sc-28303 FITC | 200 µg/ml | $336.00 | |||
cyclin B2 Antibody (A-2) PE | sc-28303 PE | 200 µg/ml | $349.00 | |||
cyclin B2 Antibody (A-2) Alexa Fluor® 488 | sc-28303 AF488 | 200 µg/ml | $364.00 | |||
cyclin B2 Antibody (A-2) Alexa Fluor® 546 | sc-28303 AF546 | 200 µg/ml | $364.00 | |||
cyclin B2 Antibody (A-2) Alexa Fluor® 594 | sc-28303 AF594 | 200 µg/ml | $364.00 | |||
cyclin B2 Antibody (A-2) Alexa Fluor® 647 | sc-28303 AF647 | 200 µg/ml | $364.00 | |||
cyclin B2 Antibody (A-2) Alexa Fluor® 680 | sc-28303 AF680 | 200 µg/ml | $364.00 | |||
cyclin B2 Antibody (A-2) Alexa Fluor® 790 | sc-28303 AF790 | 200 µg/ml | $364.00 |