CUL-4 Antibody (H-11): sc-377188

CUL-4 Antibody (H-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects CUL-4 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-CUL-4 antibody (H-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CUL-4 plays a crucial role in the ubiquitin-proteasome system, specifically in the selective degradation of proteins, which is vital for maintaining cellular homeostasis and regulating various biological processes, including cell cycle and DNA repair. CUL-4 functions as part of an E3 ubiquitin ligase complex, where CUL-4 associates with Nedd-8, a ubiquitin-like protein, to facilitate ubiquitination of target substrates. This post-translational modification is essential for controlling protein levels and activity within cells, thereby influencing processes such as cell proliferation and response to stress. CUL-4′s regulation of protein degradation underscores its importance in cellular signaling pathways and potential implications in cancer and other diseases where these pathways are disrupted.

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CUL-4 Antibody (H-11) References:

1. One ring to rule a superfamily of E3 ubiquitin ligases.  |  Tyers, M. and Willems, AR. 1999. Science. 284: 601, 603-4. PMID: 10328744
2. Cullin-3 targets cyclin E for ubiquitination and controls S phase in mammalian cells.  |  Singer, JD., et al. 1999. Genes Dev. 13: 2375-87. PMID: 10500095
3. Identification of the von Hippel-lindau tumor-suppressor protein as part of an active E3 ubiquitin ligase complex.  |  Iwai, K., et al. 1999. Proc Natl Acad Sci U S A. 96: 12436-41. PMID: 10535940
4. CUL-4 ubiquitin ligase maintains genome stability by restraining DNA-replication licensing.  |  Zhong, W., et al. 2003. Nature. 423: 885-9. PMID: 12815436
5. The Caenorhabditis elegans replication licensing factor CDT-1 is targeted for degradation by the CUL-4/DDB-1 complex.  |  Kim, Y. and Kipreos, ET. 2007. Mol Cell Biol. 27: 1394-406. PMID: 17145765
6. Overexpression of the human homologue for Caenorhabditis elegans cul-4 gene is associated with poor outcome in node-negative breast cancer.  |  Schindl, M., et al. 2007. Anticancer Res. 27: 949-52. PMID: 17465225
7. Human homologue for Caenorhabditis elegans CUL-4 protein overexpression is associated with malignant potential of epithelial ovarian tumours and poor outcome in carcinoma.  |  Birner, P., et al. 2012. J Clin Pathol. 65: 507-11. PMID: 22447918
8. Cul-4 inhibition rescues spastin levels and reduces defects in hereditary spastic paraplegia models.  |  Sardina, F., et al. 2024. Brain. 147: 3534-3546. PMID: 38551087
9. cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family.  |  Kipreos, ET., et al. 1996. Cell. 85: 829-39. PMID: 8681378
10. Identification and analysis of expression of human VACM-1, a cullin gene family member located on chromosome 11q22-23.  |  Byrd, PJ., et al. 1997. Genome Res. 7: 71-5. PMID: 9037604
11. The human homologue for the Caenorhabditis elegans cul-4 gene is amplified and overexpressed in primary breast cancers.  |  Chen, LC., et al. 1998. Cancer Res. 58: 3677-83. PMID: 9721878
12. Human CUL-1 associates with the SKP1/SKP2 complex and regulates p21(CIP1/WAF1) and cyclin D proteins.  |  Yu, ZK., et al. 1998. Proc Natl Acad Sci U S A. 95: 11324-9. PMID: 9736735

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CUL-4 Antibody (H-11) has 12 citations in a variety of scientific publications.

Primary antibodies like Anti-CUL-4 Antibody (H-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CUL-4 Antibody (H-11) Specifications

Alternate Product Name: CUL-4

Product Clone Name: H-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 536-597

Epitope Target: CUL-4 (cullin-4)

Epitope Species: human

Product Reactivity/Detection: CUL-4A and CUL-4B

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 12 citations in various scientific publications

Available Conjugates/Forms for CUL-4 Antibody (H-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin