COL1A1 (3G3): sc-293182 [ COL1A1 (C-18)의 권장하는 대체 단클론항체입니다. ]
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COL1A1 항체 (3G3)
sc-293182
100 µg/ml
$322.00
COL1A1 (3G3): m-IgG3 BP-HRP 번들
sc-550413
100 µg Ab; 40 µg BP
$361.00
at what dilution have this antibody been used?
Asked by: LGrC
Thank you for your question. What dilution we use depends on the application it is being used for, as well as sample type and secondary reagents, but here is a general guideline of starting dilutions (further titration/optimization will be required):
Western blot: 1:100
Immunofluorescence/IHC: 1:50
Most other applications, we recommend doing a titration.
Answered by: Tech Service
Date published: 2022-09-27
what is the best blocking agent for this antibody BSA or fat free milk and in which buffer
Asked by: Anonymous
Thank you for your question.
Which blocking reagent depends on which application you are using the antibody for. For Western blot we recommend 5% milk with TBS-T. For IHC and IF applications we recommend using blocking serum derived from the same species in which the secondary antibody is raised.
Rated 5 out of
5 by
David Jen from
sc-8784 quality for research.It works very well in the Western blot analysis. We recommend it to use.
Date published: 2017-07-18
Rated 5 out of
5 by
kdd246 from
Perfect functionwe use this antibody nearly one year, it works very well, and it is trustworthy.
Date published: 2016-11-28
Rated 4 out of
5 by
Hao_Xu from
Quite strong in mouse arteriesQuite strong in mouse arteries, Band of interest seen clearly plus some other bands.
Date published: 2015-09-23
Rated 5 out of
5 by
Jian from
Worked well for WB in mouseWorked well for WB in mouse.
Date published: 2015-08-21
Rated 5 out of
5 by
SM from
Worked well with murine tissueWorked well with murine tissue.
Date published: 2015-06-19
Rated 5 out of
5 by
Odinuga from
This antibody has been working well for me so farThis antibody has been working well for me so far. This is our second time ordering it and we have used it in WB and IF and obtained great results.
Date published: 2015-06-04
Rated 5 out of
5 by
Raye from
No commentNo comment
Date published: 2015-03-23
Rated 5 out of
5 by
BO from
Worked well for IF with methanolWorked well for IF with methanol-fixed HS68 cells. -SCBT QC