Date published: 2025-9-8

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COL1A1 항체 (3G3): sc-293182

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데이터 시트
  • COL1A1 항체 3G3 는 마우스 monoclonal IgG3 κ COL1A1 항체, 179간행물에 인용, 이며 100 µg/ml으로 제공합니다.
  • human 기원 COL1A1의 1021-1108 아미노산에 해당하는 재조합 단백질에 대해 생성됨
  • WB, IP, IF, IHC(P)와 ELISA으로 mouse, rat와 human origin의 Collagen α1 Type I을 검출할것을 권장합니다.
  • COL1A1 (3G3): sc-293182무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgG3 BP-HRP 는COL1A1 抗体 (3G3) 가 WB and IHC(P) 에 응용될 때 우선으로 사용하는 이차 항체 검사 시약입니다.이 시약은COL1A1 항체(3G3) 와 세트로 제공드립니다. (아래의 주문 정보를 참고해 주시길 바랍니다).

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COL1A1 항체(3G3)는 콜라겐 α1 타입 I, 즉 COL1A1 또는 타입 I 콜라겐 알파-1 사슬을 표적으로 하는 마우스 단일 클론 IgG3 카파 경쇄 항체로서, 다양한 결합 조직에서 세포 외 기질(ECM)의 중요한 구성 요소입니다. COL1A1 단일 클론 항체(3G3)는 인간 COL1A1의 아미노산 1021-1108에 해당하는 재조합 단백질에 대해 생성되며, 웨스턴 블롯(WB), 면역침전법(IP), 면역형광법(IF), 파라핀 포매 절편을 이용한 면역조직화학(IHCP), 효소결합 면역흡착 분석법(ELISA) 등의 응용을 통해 마우스, 쥐, 인간 샘플에서 COL1A1을 검출합니다. COL1A1은 주로 ECM에 위치하며, 힘줄, 피부, 뼈와 같은 조직에 인장 강도와 구조적 완전성을 제공하는 섬유상 구조를 형성합니다. ECM에 COL1A1이 존재하는 것은 조직 구조를 유지하고, 세포 부착을 촉진하며, 이동과 분화와 같은 세포 기능을 지원하는 데 필수적입니다. 또한, COL1A1은 흉터 조직 형성에 기여함으로써 상처 치유와 조직 복구에 중요한 역할을 합니다. 항-COL1A1 항체(3G3)는 비접합 형태로 제공되어, 연구자들이 콜라겐 관련 과정을 연구하는 다양한 실험 프로토콜에 이를 통합할 수 있도록 합니다. COL1A1 단일클론항체(3G3)는 세포외기질 역학 및 건강과 질병에 미치는 영향을 연구하는 연구자들에게 권장됩니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

COL1A1 항체 (3G3) 참고문헌:

  1. 콜라겐 및 뼈 강도.  |  Boskey, AL., et al. 1999. J Bone Miner Res. 14: 330-5. PMID: 10027897
  2. 검토 문서: 혈소판-콜라겐 상호작용: 막 수용체 및 세포 내 신호 경로.  |  Alberio, L. and Dale, GL. 1999. Eur J Clin Invest. 29: 1066-76. PMID: 10583456
  3. 콜라겐 매트릭스에 대한 세포 부착.  |  McCarthy, JB., et al. 1996. Biopolymers. 40: 371-81. PMID: 8765608
  4. 콜라겐 II형 우성 및 아형 에피토프에 대한 비강 내성 및 콜라겐 유발 관절염에 대한 보호.  |  Staines, NA., et al. 1997. Biochem Soc Trans. 25: 661-4. PMID: 9191176
  5. 무척추동물의 다용도 콜라겐.  |  Engel, J. 1997. Science. 277: 1785-6. PMID: 9324767
  6. 자가 면역의 동물 모델인 콜라겐 유발 관절염.  |  Myers, LK., et al. 1997. Life Sci. 61: 1861-78. PMID: 9364191
  7. 연골 콜라겐: 동물의 실험적 관절염과 인간 류마티스 질환의 발병 기전에서 콜라겐의 구조, 조직 및 역할에 대한 검토.  |  Cremer, MA., et al. 1998. J Mol Med (Berl). 76: 275-88. PMID: 9535561

주문정보

제품명카탈로그 번호 단위가격수량관심품목

COL1A1 항체 (3G3)

sc-293182
100 µg/ml
$316.00

COL1A1 (3G3): m-IgG3 BP-HRP 번들

sc-550413
100 µg Ab; 40 µg BP
$354.00

I understand that this antibody is raised against human col1a1 amino acid #1021-1108. What about the amino acid# of mouse col1a1?

Asked by: Sr99
Thank you for your question. Please see link for more information on this antibody : https://www.uniprot.org/uniprotkb/P11087/entry
Answered by: BlakeJ
Date published: 2024-04-06

Do you recommend use milk or BSA with this antibody?

Asked by: MDias
Thank you for your question. This antibody has been established with UltraCruz® Blocking Reagent: sc-516214. BSA-TBST or milk-TBST are also suitable for blocking. Depending on your particular experimental setup you may want to opt for a BSA-based blocking buffer to increase overall signal strength or a milk-based buffer for improved signal to noise ratio and lower background.
Answered by: Tech Support Europe
Date published: 2023-10-24

Is it possible to use this antibody for IHC(P) on rabbit tissue?

Asked by: XuMY
Thank you for your question. It doesn't seem COL1A1 is fully characterized in Oryctolagus cuniculus. However, there seems to be a high homology between the epitope sequence of the antibody and the predicted sequence of collagen alpha-1(I) chain of rabbit origin, accession# XP_017204320. It may be possible that this antibody may detect your target but we cannot guarantee it since the sequence has not been fully characterized.
Answered by: Santa Cruz TS
Date published: 2021-07-10

Would this antibody work on COL1 of porcine origin?

Asked by: KaraThrace
Thank you for your question. This antibody has not yet been tested in porcine and so that is not a recommended species at this time. We are continuously gathering data to show on our website. Check back for potential updates! If you have any further questions, please contact Technical Service.
Answered by: Technical Service 3
Date published: 2020-11-12

Would this ABY work for western analysis of the purified rat-tail collagen used for cell culture?

Asked by: Monger
Thank you for your question. We haven't tested this antibody using purified rat-tail collagen used for cell culture, so we do not know if it would work on that protein.
Answered by: Technical Support
Date published: 2022-06-01

I checked col1a1 in mice kidney extract through WB.While Band positions were detected from 52 kDa to 76 kDa, not in the same location as the protocol. Even if it is a decomposed product, the molecular weight is small. Please tell me What is it.

Asked by: MHTK
Thank you for your question. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2020-07-23

hello. I have an one question about this antibody. Can this antibody even recognize pro collagen type 1? I'm wondering if this ab can distinguish collagen fiber and pro collagen.

Asked by: finalb2
Thank you for your question. Based on the epitope mapping, this antibody should be able to recognize both pro collagen alpha1 type I, and the mature protein. We have not tested the antibody to determine if it can distinguish between collagen fiber and pro collagen, but we would recommend you review some of the product citations using this antibody for more information about how the antibody is being used.
Answered by: Technical Support
Date published: 2020-03-31

I used this antibody to check col1a1 in mice kidney extract through WB.While the reasult showed a lot of non-specific bands and i couldn't comfirm which is the col1a1. 1st dil. 1:1000. 2nd anti-mouse lgG dil.1:5000. Could u provide a positive con?

Asked by: hhx112
Thank you for your question. Please contact your local distributor for assistance in troubleshooting this antibody. They will be happy to help you!
Answered by: Tech Service
Date published: 2021-03-19
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Rated 4 out of 5 by from Buon funzionamento su FFPETestato su cute canina 1:150, buon funzionamento, un po' di fondo.
Date published: 2025-05-12
Rated 5 out of 5 by from Very good bands in Western blottingBoth mature collagen and procollagen can be detected, although it takes some trickery to solubilize samples for collagen-specific protocol.
Date published: 2024-08-18
Rated 2 out of 5 by from Reduced visibility and aspecificity in WBI tested this antibody on NHDF human dermal fibroblasts, to evaluate the modulation of collagen under the modulators of the Wound healing process. However, in its application for western blot, using the 1:1000 dilution, the antibody highlights a non-specific around 50 kDa, the intensity of which reduces the visibility of the isoform for which this antibody should be specific. I recommend cutting the membrane properly.
Date published: 2023-03-27
Rated 4 out of 5 by from Col1A1 in conditioned mediaWe used the Col1A1 antibody in human lung fibroblast culture treated with TGF-b1. Supernatant were collected 24, hours. Slot blot analysis revealed increase in Col1A1 expression.
Date published: 2022-06-06
Rated 4 out of 5 by from Bene in wbHo provato questo anticorpo in wb (1:1000) su spinal cord di topo ed ha funzionato molto bene! Ottimo anticorpo
Date published: 2020-10-05
Rated 5 out of 5 by from Good for WBIt's useful for detecting both Pro-collagen I and matured collagen I.
Date published: 2020-09-04
Rated 5 out of 5 by from good for immunohistochemistryImmunohistochemistry: Paraffin kidney section, immune infiltrate Antibody dilution: 1:50 Antigen retrieval in citrate buffer pH6 Comment: Good signal
Date published: 2020-08-10
Rated 1 out of 5 by from No encontramos señal.WB muy sucio; imposible detectar señal de ñaproteina.
Date published: 2020-05-09
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COL1A1 항체 (3G3) is rated 4.1 out of 5 by 29.
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