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cathepsin D Antibody (D-7) - Western Blotting - Image 283620
카텝신 D 항체(D-7): sc-377299. SK-BR-3(A), NIH/3T3(B), RAW 264.7(C) 및 AMJ2-C11(D) 전체 세포 용해물에서 카텝신 D 발현의 웨스턴 블롯 분석.
카텝신 D 항체(D-7): sc-377299. SK-BR-3(A), NIH/3T3(B), RAW 264.7(C) 및 AMJ2-C11(D) 전체 세포 용해물에서 카텝신 D 발현의 웨스턴 블롯 분석.

cathepsin D 항체 (D-7): sc-377299

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데이터 시트
  • cathepsin D 항체 D-7 는 마우스 monoclonal IgM κ cathepsin D 항체, 49간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • 항원결정부는 human origin의 cathepsin D의 내부에 위치한 383-411의 아미노산에 해당됩니다.
  • WB, IP, IF, IHC(P)와 ELISA으로 mouse, rat와 human origin의 cathepsin D을 검출할것을 권장합니다.
  • IP에 사용가능한 agarose결합제품; HRP/b> for WB, IHC(P) and ELISA; IF, IHC(P) 와 FCM에 사용가능한 phycoerythrin/b>결합제품, FITC결합제품, Alexa Fluor® 488/b>결합제품 와 Alexa Fluor® 647/b> 결합제품 이 있습니다.
  • cathepsin D (D-7): sc-377299의 무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgGκ BP-HRP 는 cathepsin D 항체 (D-7) 가 WB and IHC(P) 에 응용될 때 우선으로 사용하는 이차 항체 검사 시약입니다. 이 시약은cathepsin D 항체인 (D-7)와 세트로 제공드립니다。 (아래의 주문 정보를 참고해 주시길 바랍니다)。기타m-IgGκ BP 커플링제는，작은쥐 IgG 결합 단백질완정한 리스트를 참고 해 주시길 바랍니다.

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데이터 시트 및 프로토콜
연구 정보

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cathepsin 6, cathepsin A, cathepsin B, cathepsin C, cathepsin D, cathepsin E, cathepsin H, cathepsin K, cathepsin L 와cathepsin O를 위한 Cathepsin 항체

켑신 D 항체(D-7)는 마우스, 쥐 및 인간 유래의 켑신 D 단백질을 WB, IP, IF, IHC(P) 및 ELISA로 검출하는 IgM κ 마우스 단일 클론 켑신 D 항체(CTSD 항체로도 지정됨)입니다. 켑신 D 항체(D-7)는 비접합 항-카텝신 D 항체 형태와 아가로스, HRP, PE, FITC 및 여러 Alexa Fluor® 접합체를 포함한 여러 접합 형태의 항-카텝신 D 항체 형태로 제공됩니다. 카텝신 계열의 단백질 분해 효소에는 여러 가지 다양한 종류의 프로테아제가 포함되어 있습니다. 시스테인 프로테아제 계열은 켑신 B, L, H, K, S, O로 구성되며, 아스파틸 프로테아제 계열은 켑신 D와 E로 구성되며, 켑신 G는 세린 프로테아제 계열에 속합니다. 대부분의 켑신은 리소좀형이며 각각 세포 대사에 관여하여 펩타이드 생합성 및 단백질 분해와 같은 다양한 사건에 참여합니다. 또한 켑신은 일부 단백질 전구체를 절단하여 조절 펩타이드를 방출할 수도 있습니다. 켑신 D 유전자의 프로모터 영역에는 5개의 Sp1 결합 부위와 4개의 AP-2 결합 부위가 있습니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

주문정보

제품명카탈로그 번호 단위가격수량관심품목

cathepsin D 항체 (D-7)

sc-377299
200 µg/ml
$316.00

cathepsin D (D-7): m-IgGκ BP-HRP 번들

sc-550757
200 µg Ab; 40 µg BP
$354.00

cathepsin D 항체 (D-7) AC

sc-377299 AC
500 µg/ml, 25% agarose
$416.00

cathepsin D 항체 (D-7) Alexa Fluor® 488

sc-377299 AF488
200 µg/ml
$357.00

cathepsin D 항체 (D-7) Alexa Fluor® 647

sc-377299 AF647
200 µg/ml
$357.00

cathepsin D 항체 (D-7) FITC

sc-377299 FITC
200 µg/ml
$330.00

cathepsin D 항체 (D-7) HRP

sc-377299 HRP
200 µg/ml
$316.00

cathepsin D (D-7) 중화펩타이드

sc-377299 P
100 µg/0.5 ml
$68.00

cathepsin D 항체 (D-7) PE

sc-377299 PE
200 µg/ml
$343.00

What is recommendable for a WesternBlot, using cathepsin D Antibody (D-7): sc-377299 and a secondary antibody or use directly cathepsin D Antibody (D-7): sc-377299 conjugated with HRP? Thanks

Asked by: CUES
Thank you for your question. It is by personal preference to reduce the number of steps on your protocol we recommend using the HRP conjugated form. https://www.scbt.com/resources/protocols/western-immuno-blotting
Answered by: BlakeJ
Date published: 2023-08-18

Does it work in pig tissue? Thanks.

Asked by: YMC39
Thank you for your question. We validate this antibody for mouse, rat and human. There is a 78% homology in pig. We only honor our warranty if the homology is 95% or higher.
Answered by: BlakeJ
Date published: 2023-03-30

Does this work by immunofluorescence in mouse cells? Is there a recommended dilution for this application? Thank you.

Asked by: scienceguy
Anti-cathepsin D Antibody (D-7): sc-377299 is recommended for detection of cathepsin D of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. For immunofluorescence, the recommended starting dilution is 1:50, with a dilution range between 1:50-1:500.
Answered by: Tech Support Europe
Date published: 2021-08-25

I will use this antibody (sc-377299) in western blot. Can you suggest second antibody (HRP conjugated) for me? Thanks.

Asked by: Researcherman
Thank you for your question. As secondary antibody with HRP conjugate we recommend m-IgGκ BP-HRP: sc-516102
Answered by: Tech Support Europe
Date published: 2021-07-14

What is the best reagent to dilute this antibody for Western Blotting?

Asked by: MZOIA
Thank you for your question. We recommend using our TBS Blotto A: sc-2333 (https://www.scbt.com/scbt/product/tbs-blotto-a) which is 5% nonfat milk + 1X TBS + 0.05% Tween-20. This is the recommended buffer for blocking, as well as primary and secondary antibody incubations for Western blotting.
Answered by: Technical Support
Date published: 2017-11-08

cathepsin D (D-7): sc-377299 is a mouse IgM, can I use an anti-mouse IgG secondary antibody for detection?

Asked by: cjMara
We recommend using an isotype-specific anti-mouse IgM secondary antibody for detection of this mouse IgM primary antibody. Anti-mouse IgG secondary antibodies may work, due to the similar structures of IgG and IgM, but they are not ideally suited.
Answered by: Technical Support
Date published: 2017-02-02
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Rated 3 out of 5 by from This antibosy works with western blottingThis antibody works with western blotting with SAS cells lysate.
Date published: 2020-06-12
Rated 4 out of 5 by from It works wellWe checked this antibody on lysates from immortalized human proximal tubular epithelial cells by Western blot. It detects a single band (around 48-50 kDa, presumably the immature form) with no background signals, after overnight incubation (1:500) at 4°C. We were unable to detect the mature form of Cathepsin D (35 kDa) in these cells upon these experimental conditions.
Date published: 2019-06-12
Rated 5 out of 5 by from works very well for mouse fat tissueI did 1/500 dilution for 4 degree overnight incubation, which gives me expected band, upper band between 40-55 kDa, around 52Kda or 46 kDa, I am not sure. But it also can detect the mouse IgG light chain, the bottom band
Date published: 2017-12-21
Rated 3 out of 5 by from Working antibody in WBThis antibody works ok in WB with human and mouse samples. There are quite a few non-specific bands.
Date published: 2017-10-18
Rated 5 out of 5 by from it's excitingWe made an experiment with cathepsin D antibody and found it very useful. This antibody has very good activity and a demonstrable effect.
Date published: 2017-10-18
Rated 1 out of 5 by from It doesn't work in IF in rat 832/13 ins1 cellsPFA fixed, permeabilized with Triton-X10. Used at 1:50 for IF
Date published: 2017-10-15
Rated 5 out of 5 by from good featuresuse in NB4 cells ,the trips is very abvious,thank you for your free sample
Date published: 2017-09-06
Rated 5 out of 5 by from Works greatWorks great with no background signals on western blot at 1:200 in mouse cell line
Date published: 2017-08-03
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cathepsin D 항체 (D-7) is rated 4.4 out of 5 by 20.
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