Date published: 2025-9-7

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caspase-1 항체 (D-3): sc-392736

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데이터 시트
  • caspase-1 항체 D-3 는 마우스 monoclonal IgG1 κ caspase-1 항체, 66간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • human origin의 caspase-1에서 C-terminus에 위치한 367-391의 아미노산사이에 위치한 항원결정부와 특정결합합니다.
  • caspase-1 항체 (D-3)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 caspase-1 를 감지하는 데에 추천한다.
  • 항-caspase-1 항체(D-3)는 IP용 agarose와 결합되어 이용 가능하며, WB, IHC(P), ELISA용 HRP와 결합되어 이용 가능하며, IF, IHC(P), FCM용 phycoerythrin 또는 FITC와 결합되어 이용 가능합니다.
  • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • caspase-1 (D-3): sc-392736무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 는 caspase-1 항체 (D-3) 가 WB and IHC(P) 에 응용될 때 우선으로 사용하는 이차 항체 검사 시약입니다.이 시약은caspase-1 항체(D-3) 와 세트로 제공드립니다. (아래의 주문 정보를 참고해 주시길 바랍니다).

빠른 링크

더보기

caspase-1 항체(D-3)는 마우스, 쥐 및 인간 유래의 카스파제-1 단백질을 WB, IP, IF, IHC(P) 및 ELISA로 검출하는 IgG1 κ 마우스 모노클로날 카스파제-1 항체(CASP1 항체로도 지정됨)입니다. 카스파제-1 항체(D-3)는 비공액형 항카스파제-1 항체 형태와 아가로스, HRP, PE, FITC 및 다중 Alexa Fluor® 접합체를 포함한 여러 공액형 항카스파제-1 항체 형태 모두로 사용할 수 있습니다. 원래 ICE(IL-1 전환 효소)로 지정된 카스파제-1은 큰 프로도메인을 가진 카스파제 그룹에 속하는 효소입니다. 카스파제-1은 전구체 형태를 생물학적으로 활성인 전 염증성 사이토카인으로 단백질 분해 분해하여 인터루킨 IL-1β 및 인터루킨18(IL-18)의 성숙을 촉진합니다. (p20/p10)2 사면체인 활성 카스파제-1은 전구체 IL-1의 절단과 일부 세포주에서 세포 사멸을 유도하는 데 필요하고 충분합니다. 고도로 보존된 카스파제 계열은 세포체와 핵의 구조적 해체, 게놈 DNA의 단편화, 조절 단백질의 파괴, 다른 세포 사멸 촉진 분자의 전파 등 세포 사멸의 많은 형태학적 및 생화학적 특징을 매개합니다. 인간 카스파제-1 유전자는 염색체 2q14에 매핑되어 있으며 간, 심장, 골격근 신장 및 고환에서 발현되는 세포질 단백질을 암호화합니다. 카스파제-1은 염증, 패혈성 쇼크, 상처 치유 및 특정 백혈병의 성장과 같은 기타 상황에 관여하는 것으로 알려져 있습니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

주문정보

제품명카탈로그 번호 단위가격수량관심품목

caspase-1 항체 (D-3)

sc-392736
200 µg/ml
$316.00

caspase-1 (D-3): m-IgG Fc BP-HRP 번들

sc-530332
200 µg Ab; 10 µg BP
$354.00

caspase-1 (D-3): m-IgGκ BP-HRP 번들

sc-523771
200 µg Ab, 40 µg BP
$354.00

caspase-1 (D-3): m-IgG1 BP-HRP 번들

sc-543994
200 µg Ab; 20 µg BP
$354.00

caspase-1 항체 (D-3) AC

sc-392736 AC
500 µg/ml, 25% agarose
$416.00

caspase-1 항체 (D-3) HRP

sc-392736 HRP
200 µg/ml
$316.00

caspase-1 항체 (D-3) FITC

sc-392736 FITC
200 µg/ml
$330.00

caspase-1 항체 (D-3) PE

sc-392736 PE
200 µg/ml
$343.00

caspase-1 항체 (D-3) Alexa Fluor® 488

sc-392736 AF488
200 µg/ml
$357.00

caspase-1 항체 (D-3) Alexa Fluor® 546

sc-392736 AF546
200 µg/ml
$357.00

caspase-1 항체 (D-3) Alexa Fluor® 594

sc-392736 AF594
200 µg/ml
$357.00

caspase-1 항체 (D-3) Alexa Fluor® 647

sc-392736 AF647
200 µg/ml
$357.00

caspase-1 항체 (D-3) Alexa Fluor® 680

sc-392736 AF680
200 µg/ml
$357.00

caspase-1 항체 (D-3) Alexa Fluor® 790

sc-392736 AF790
200 µg/ml
$357.00

caspase-1 (D-3) 중화펩타이드

sc-392736 P
100 µg/0.5 ml
$68.00

I am wondering if we can detect both pro and cleaved caspase 1 with this sc-392736 antibody as I always get two bands every time with 1: 750 dilution.

Asked by: Nandy
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2023-01-11

what is the volume?

Asked by: ailin
Thank you for your question. The antibody comes as 200 µg in 1 ml.
Answered by: Tech Support Europe
Date published: 2021-08-12

Why do I always get a positive band at 72 kD?

Asked by: Lorrainedd
Thank you for your question. Please contact technical service for troubleshooting advice on your experiment.
Answered by: Tech Support Europe
Date published: 2022-07-30

What is the difference between D-3 and D-4? Do these detect cleaved caspase 1 as well?

Asked by: cindyha_uot
Thank you for your question. These are both mouse monoclonal antibodies, but they are have different isotypes, and are raised against different antigens. The caspase-1 Antibody (D-3): sc-392736 is raised against an epitope mapping between amino acids 367-391 (human origin), and caspase-1 p20 Antibody (D-4): sc-398715 is raised against an epitope mapping between amino acids 259-294 (mouse origin). Both antibodies are expected to detect cleaved caspase, however (D-3) should recognize the p10 subunit, and (D-4) should recognize the p20 subunit. Please contact our Technical Service Department for help in choosing the best antibody for your research.
Answered by: Technical Support
Date published: 2020-03-11

I was wondering how many tests can be run with one kit of the following Ab: caspase-1 Antibody (D-3): sc-392736

Asked by: Anonymous5
Thank you for your question. We provide 200 µg in 1 mL. The number of tests that can be performed using this antibody depends on your application, dilution and final working volumes.
Answered by: Technical Support
Date published: 2023-07-31

I used SC-1780 (caspase-1 Ab) to detect cleaved-caspase-1 (p20) polyclonal antibody. I would like to change the Ab to SC-392736 monoclonal Ab. But there is not any comment for detection of cleaved-caspase-1 (p20) protein. Can I detect using SC-392736?

Asked by: Jeong H
Thank you for your question. We would not recommend this mouse monoclonal, caspase-1 Antibody (D-3): sc-392736, for detection of the cleaved caspase-1 p20 subunit. This antibody is specific for an epitope mapping between amino acids 367-391 near the C-terminus of caspase-1 of human origin. And according to Uniprot, the p20 subunit corresponds to a different region between amino acids 120-297. http://www.uniprot.org/uniprot/P29466#ptm_processing
Answered by: Technical Support
Date published: 2023-07-04

What application is the blocking peptide sc-392736 P appropriate for?

Asked by: TinTin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
  • y_2025, m_8, d_27, h_5CST
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Rated 3 out of 5 by from Didnt work for wetsern blotDidnt work for wetsern blot and there is a batch consistency with this antibody
Date published: 2019-12-04
Rated 4 out of 5 by from Good, decent antibodyThis antibody detected a strong 45 kDa band, but the background was too high. We even used 5% milk to block as instructed. Overall, the band could be seen even though high background.
Date published: 2018-03-14
Rated 5 out of 5 by from Worked well for western blotDetected good protein band at 1:200 dilution. no background or nonspecific bands
Date published: 2018-02-07
Rated 3 out of 5 by from Clear bandsI saw clear bands at 48kda and 25kda however I was looking for bands at 45, 20, and 10. I'm not sure if I'll be able to use this antibody (using mouse lung homogenates).
Date published: 2017-11-30
Rated 4 out of 5 by from wb效果不错我之前申请了caspase1抗体的试用装,感觉WB效果很好,但是稀释比例还需要进一步摸索,感觉还有变化的空间。
Date published: 2017-09-13
Rated 5 out of 5 by from perfectvery nice,it work well in WB at 1:1000,rat platelet.
Date published: 2017-09-05
Rated 3 out of 5 by from Worked well for cell lysates (human)Detected procaspase 1 bands at 1:1000 dilution in 2% milk in TBST. The signal was week and lots of non specific bands were observed
Date published: 2017-08-15
Rated 5 out of 5 by from good result on WBThe antibody caspase-1 works well in WB at 1:500, rat articular chondrocytes cells, protein bands are clear
Date published: 2017-08-10
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caspase-1 항체 (D-3) is rated 4.3 out of 5 by 18.
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