BPDE-DNA Antibody (5D11): sc-52625

BPDE-DNA Antibody (5D11) is a mouse monoclonal IgG2a kappa light chain antibody that detects BPDE-DNA by immunofluorescence (IF) and immunohistochemistry with paraffin-embedded sections (IHCP). Anti-BPDE-DNA antibody (5D11) is available in both non-conjugated and multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates. Benzopyrene-7,8-diol-9,10-epoxide (BPDE) is a potent mutagen and carcinogen derived from the incomplete combustion of organic materials, commonly found in coal tar, tobacco smoke, and charbroiled foods. The significance of BPDE lies in its ability to form covalent bonds with DNA, particularly at the N2 position of guanine nucleobases, leading to DNA intercalation and distortion. This interaction can result in increased micronuclei formation and apoptosis, contributing to the development of various cancers, especially lung cancer. Understanding the mechanisms of BPDE′s action is crucial for developing strategies to mitigate its harmful effects and for advancing cancer research.

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BPDE-DNA Antibody (5D11) References:

1. Immunohistochemical analysis of polycyclic aromatic hydrocarbon-DNA adducts in breast tumor tissue.  |  Santella, RM., et al. 2000. Cancer Lett. 154: 143-9. PMID: 10806302
2. Benzo(a)pyrene diolepoxide (BPDE)-DNA adduct levels in leukocytes of smokers in relation to polymorphism of CYP1A1, GSTM1, GSTP1, GSTT1, and mEH.  |  Lodovici, M., et al. 2004. Cancer Epidemiol Biomarkers Prev. 13: 1342-8. PMID: 15298956
3. XP-A cells complemented with Arg228Gln and Val234Leu polymorphic XPA alleles repair BPDE-induced DNA damage better than cells complemented with the wild type allele.  |  Porter, PC., et al. 2005. DNA Repair (Amst). 4: 341-9. PMID: 15661657
4. Protection by quercetin and quercetin-rich fruit juice against induction of oxidative DNA damage and formation of BPDE-DNA adducts in human lymphocytes.  |  Wilms, LC., et al. 2005. Mutat Res. 582: 155-62. PMID: 15781220
5. DNA polymerase kappa is specifically required for recovery from the benzo[a]pyrene-dihydrodiol epoxide (BPDE)-induced S-phase checkpoint.  |  Bi, X., et al. 2005. J Biol Chem. 280: 22343-55. PMID: 15817457
6. Membrane assembly of lactose permease of Escherichia coli.  |  Yamato, I. 1992. J Biochem. 111: 444-50. PMID: 1618733
7. Age-related changes in oxidative DNA damage and benzo(a)pyrene diolepoxide-I (BPDE-I)-DNA adduct levels in human stomach.  |  Lee, BM., et al. 2005. J Toxicol Environ Health A. 68: 1599-610. PMID: 16195216
8. Detection of BPDE-DNA adducts in human umbilical cord blood by LC-MS/MS analysis.  |  Guo, L., et al. 2019. J Food Drug Anal. 27: 518-525. PMID: 30987723
9. BPDE-DNA adduct formation and alterations of mRNA, protein, and DNA methylation of CYP1A1, GSTP1, and GSTM1 induced by benzo[a]pyrene and the intervention of aspirin in mice.  |  Liu, A., et al. 2023. Environ Sci Pollut Res Int. 30: 106549-106561. PMID: 37730975
10. BaP/BPDE exposure causes human trophoblast cell dysfunctions and induces miscarriage by up-regulating lnc-HZ06-regulated IL1B.  |  Guo, J., et al. 2024. J Hazard Mater. 476: 134741. PMID: 38991640
11. Monoclonal antibodies to DNA modified by a benzo[a]pyrene diol epoxide.  |  Santella, RM., et al. 1984. Carcinogenesis. 5: 373-7. PMID: 6423306
12. A sensitive color ELISA for detecting polycyclic aromatic hydrocarbon-DNA adducts in human tissues.  |  Mumford, JL., et al. 1996. Mutat Res. 359: 171-7. PMID: 8618549

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-BPDE-DNA Antibody (5D11) has 11 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

BPDE-DNA Antibody (5D11) Specifications

Alternate Product Name: BPDE-DNA

Product Clone Name: 5D11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids:

Epitope Target: BPDE-I-G

Epitope Species: n/a

Product Reactivity/Detection: BPDE-DNA

Species Reactivity/Detection: NA

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 11 citations in various scientific publications

Available Conjugates/Forms for BPDE-DNA Antibody (5D11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin