ZNF526 CRISPR/Cas9 KO Plasmid (m): sc-431684
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ZNF526 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ZNF526 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZNF526 CRISPR/Cas9 KO Plasmid (m) | sc-431684 | 20 µg | $397.00 |
Overview
Zfp526 encodes the mouse zinc finger protein ZNF526, a putative nuclear DNA-binding factor implicated in transcriptional regulation through C2H2-type zinc finger–mediated interactions with regulatory elements. As with many KRAB-associated zinc finger proteins, ZNF526 is expected to participate in chromatin-dependent gene silencing and modulation of lineage-specific transcriptional programs that influence cell state and differentiation. Altered expression or dysregulation of zinc finger transcription factors can perturb epigenetic control networks and downstream signaling pathways, providing a framework to study gene regulatory mechanisms relevant to developmental phenotypes and disease-associated transcriptional imbalance. Functional interrogation of Zfp526 supports investigations into how transcription factor–chromatin interfaces shape gene expression and cellular homeostasis in mouse model systems.
ZNF526 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Zfp526 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Zfp526 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Zfp526 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ZNF526 protein expression.
This CRISPR knockout system enables efficient generation of Zfp526-deficient cell models for investigation of ZNF526 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Zfp526 exon(s) critical for ZNF526 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Zfp526 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ZNF526 CRISPR/Cas9 KO Plasmid (m) and ZNF526 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Zfp526 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ZNF526 HDR Plasmid (m) and ZNF526 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Zfp526 homology arms to support homology-directed repair at defined Zfp526 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.