Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
XTP3TPA CRISPR/Cas9 KO Plasmid (h): sc-407430
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- XTP3TPA CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the XTP3TPA genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: XTP3TPA Antibody (B-6): sc-398501
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
XTP3TPA CRISPR/Cas9 KO Plasmid (h) | sc-407430 | 20 µg | $397.00 |
Overview
DCTPP1 encodes XTP3TPA, a cytosolic dCTP pyrophosphatase that hydrolyzes noncanonical deoxynucleoside triphosphates to help maintain balanced dNTP pools and nucleotide quality control. By limiting incorporation of aberrant nucleotides into DNA, XTP3TPA supports genome stability, replication fidelity, and coordination of DNA repair and replication stress responses. Altered nucleotide metabolism and dNTP pool imbalance are common features of proliferative states and tumor biology, making DCTPP1 a useful node for studying links between nucleotide sanitization, oxidative damage, and mutagenesis. Functional interrogation of DCTPP1 can inform mechanisms connecting metabolic rewiring to DNA damage signaling and cell cycle control in human cells.
XTP3TPA CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the DCTPP1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the DCTPP1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the DCTPP1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish XTP3TPA protein expression.
This CRISPR knockout system enables efficient generation of DCTPP1-deficient cell models for investigation of XTP3TPA signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting DCTPP1 exon(s) critical for XTP3TPA function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple DCTPP1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by XTP3TPA CRISPR/Cas9 KO Plasmid (h) and XTP3TPA CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the DCTPP1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by XTP3TPA HDR Plasmid (h) and XTP3TPA HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by DCTPP1 homology arms to support homology-directed repair at defined DCTPP1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.