Wnt-1 Antibody (E-10): sc-514531

Wnt-1 Antibody (E-10) is a mouse monoclonal IgM antibody that detects Wnt-1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-Wnt-1 antibody (E-10) is available as a non-conjugated format. Wnt-1, a member of the highly conserved Wnt gene family, plays a crucial role in regulating cellular growth and differentiation, making Wnt-1 vital for various developmental processes. Wnt-1 is a cysteine-rich secreted glycoprotein that associates with cell membranes and is essential for normal development of the embryonic nervous system. Wnt-1 expression is typically restricted to the embryonic neural tube and adult spermatids, highlighting Wnt-1′s importance in developmental biology. Aberrant Wnt-1 expression in mammary tissue has been linked to hyperplasia and tumorigenic progression, underscoring Wnt-1′s role in cancer biology. Additionally, Wnt-1 interacts with β-catenin, a key player in cellular adhesion and a downstream target in the Wnt signaling pathway, which further emphasizes Wnt-1′s significance in both normal and pathological processes. Wnt family members, including Wnt-1, interact with other signaling pathways, such as Sonic hedgehog (Shh), to induce myogenesis in somitic tissue, illustrating the complex regulatory networks in which Wnt-1 is involved.

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Wnt-1 Antibody (E-10) References:

1. Inhibition by Wnt-1 or Wnt-3a of nerve growth factor-induced differentiation of PC12 cells is reversed by bisindolylmaleimide-I but not by several other PKC inhibitors.  |  Chou, AH. and Howard, BD. 2002. Oncogene. 21: 6348-55. PMID: 12214275
2. Wnt genes.  |  Nusse, R. and Varmus, HE. 1992. Cell. 69: 1073-87. PMID: 1617723
3. Wnt-1 dishevelled signaling functionally links calcium-calmodulin-dependent protein kinase II and cardiac dysfunction.  |  Lin, J. and Kirshenbaum, LA. 2015. Hypertension. 65: 287-8. PMID: 25489063
4. MiR-130a-3p suppresses colorectal cancer growth by targeting Wnt Family Member 1 (WNT1).  |  Song, GL., et al. 2021. Bioengineered. 12: 8407-8418. PMID: 34657551
5. Wnt family member 1 (Wnt1) overexpression-induced M2 polarization of microglia alleviates inflammation-sensitized neonatal brain injuries.  |  Gao, J., et al. 2022. Bioengineered. 13: 12409-12420. PMID: 35603707
6. Association of Membranous WNT-1 and Nuclear mTOR with Endometrial Cancer Grade.  |  Pietrus, M., et al. 2023. Int J Mol Sci. 24: PMID: 37176048
7. Combinatorial signaling by Sonic hedgehog and Wnt family members induces myogenic bHLH gene expression in the somite.  |  Münsterberg, AE., et al. 1995. Genes Dev. 9: 2911-22. PMID: 7498788
8. Biochemical analysis of murine Wnt proteins reveals both shared and distinct properties.  |  Burrus, LW. and McMahon, AP. 1995. Exp Cell Res. 220: 363-73. PMID: 7556445
9. Beta-catenin: a common target for the regulation of cell adhesion by Wnt-1 and Src signaling pathways.  |  Hinck, L., et al. 1994. Trends Biochem Sci. 19: 538-42. PMID: 7846766
10. Differential transformation of mammary epithelial cells by Wnt genes.  |  Wong, GT., et al. 1994. Mol Cell Biol. 14: 6278-86. PMID: 8065359
11. Properties of Wnt-1 protein that enable cell surface association.  |  Schryver, B., et al. 1996. Oncogene. 13: 333-42. PMID: 8710372
12. Regulation of complexed and free catenin pools by distinct mechanisms. Differential effects of Wnt-1 and v-Src.  |  Papkoff, J. 1997. J Biol Chem. 272: 4536-43. PMID: 9020180

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Wnt-1 Antibody (E-10) has 25 citations in a variety of scientific publications.

Primary antibodies like Anti-Wnt-1 Antibody (E-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Wnt-1 Antibody (E-10) Specifications

Alternate Product Name: Wnt-1

Product Clone Name: E-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 41-65

Epitope Target: Wnt-1

Epitope Species: human

Product Reactivity/Detection: Wnt-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 25 citations in various scientific publications

Available Conjugates/Forms for Wnt-1 Antibody (E-10): P: Blocking Peptide