Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
USF-1 CRISPR/Cas9 KO Plasmid (m): sc-423628
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- USF-1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the USF-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: USF-1 Antibody (G-2): sc-390027
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
USF-1 CRISPR/Cas9 KO Plasmid (m) | sc-423628 | 20 µg | $397.00 |
Overview
Upstream transcription factor 1 (USF-1), encoded by the mouse Usf1 gene, is a basic helix–loop–helix leucine zipper transcription factor that binds E-box motifs to coordinate gene expression programs controlling metabolism, growth, and stress responses. USF-1 integrates signaling inputs to regulate transcriptional networks involved in lipid and glucose homeostasis, mitochondrial function, and cell-cycle progression, often through interactions with co-regulators and chromatin-modifying complexes. In murine systems, altered Usf1 activity has been associated with dysregulated metabolic phenotypes and inflammatory signaling that are relevant to studies of cardiometabolic traits and hepatic gene regulation. As a broadly expressed transcriptional regulator, USF-1 is frequently examined for its roles in transcriptional control, enhancer–promoter communication, and pathway crosstalk underlying complex disease susceptibility.
USF-1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Usf1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Usf1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Usf1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish USF-1 protein expression.
This CRISPR knockout system enables efficient generation of Usf1-deficient cell models for investigation of USF-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Usf1 exon(s) critical for USF-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Usf1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by USF-1 CRISPR/Cas9 KO Plasmid (m) and USF-1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Usf1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by USF-1 HDR Plasmid (m) and USF-1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Usf1 homology arms to support homology-directed repair at defined Usf1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.