TRAP-α Antibody (C-8): sc-373916
- TRAP-α Antibody (C-8) is a mouse monoclonal IgG1 κ TRAP-α antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 1-286 representing full length TRAP-α of human origin
- TRAP-alpha Antibody (C-8) is recommended for detection of TRAP-α of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-TRAP-alpha Antibody (C-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for TRAP-α Antibody (C-8) for WB and IHC(P) applications. These reagents are now offered in bundles with TRAP-α Antibody (C-8) (see ordering information below).
TRAP-α Antibody (C-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects TRAP-alpha of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). TRAP-α Antibody (C-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TRAP-alpha, also known as SSR1, plays a crucial role in the translocon-associated protein complex, which is essential for proper endoplasmic reticulum (ER) functioning. TRAP-alpha is primarily located in the ER membrane, where TRAP-alpha is involved in ER resident protein retention and protein translocation regulation across the ER membrane via association with the Sec61 translocon. TRAP-alpha contributes to maintaining cellular homeostasis and protein quality control, particularly during ER stress, when TRAP-alpha is upregulated through the XBP-1/IRE1α pathway to facilitate ER-associated degradation (ERAD). The TRAP complex, which includes TRAP-α, TRAP-β, TRAP-γ, and TRAP-δ, ensures that only properly folded proteins exit the ER, thereby preventing misfolded protein accumulation that can lead to cellular dysfunction and disease.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
TRAP-α Antibody (C-8) References:
- Oligomeric complexes involved in translocation of proteins across the membrane of the endoplasmic reticulum. | Wang, L. and Dobberstein, B. 1999. FEBS Lett. 457: 316-22. PMID: 10471800
- The Translocon-Associated Protein beta (TRAPbeta) in zebrafish embryogenesis. I. Enhanced expression of transcripts in notochord and hatching gland precursors. | Mangos, S., et al. 2000. Mol Cell Biochem. 215: 93-101. PMID: 11204460
- Substrate-specific function of the translocon-associated protein complex during translocation across the ER membrane. | Fons, RD., et al. 2003. J Cell Biol. 160: 529-39. PMID: 12578908
- Cloning and molecular characterization of a human ortholog of Monodelphis TRAPD in ultraviolet B-induced melanoma. | Wang, Z. and VandeBerg, JL. 2004. Melanoma Res. 14: 107-14. PMID: 15057039
- Architecture of the ribosome-channel complex derived from native membranes. | Ménétret, JF., et al. 2005. J Mol Biol. 348: 445-57. PMID: 15811380
- Mutation in the Trapalpha/Ssr1 gene, encoding translocon-associated protein alpha, results in outflow tract morphogenetic defects. | Mesbah, K., et al. 2006. Mol Cell Biol. 26: 7760-71. PMID: 17015483
- Simultaneous induction of the four subunits of the TRAP complex by ER stress accelerates ER degradation. | Nagasawa, K., et al. 2007. EMBO Rep. 8: 483-9. PMID: 17380188
- A tetrameric complex of membrane proteins in the endoplasmic reticulum. | Hartmann, E., et al. 1993. Eur J Biochem. 214: 375-81. PMID: 7916687
- Genomic organization of two novel genes on human Xq28: compact head to head arrangement of IDH gamma and TRAP delta is conserved in rat and mouse. | Brenner, V., et al. 1997. Genomics. 44: 8-14. PMID: 9286695
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TRAP-α Antibody (C-8) | sc-373916 | 200 µg/ml | $322.00 | |||
TRAP-α Antibody (C-8): m-IgG Fc BP-HRP Bundle | sc-529608 | 200 µg Ab; 10 µg BP | $361.00 | |||
TRAP-α Antibody (C-8): m-IgGκ BP-HRP Bundle | sc-522554 | 200 µg Ab, 40 µg BP | $361.00 | |||
TRAP-α Antibody (C-8) AC | sc-373916 AC | 500 µg/ml, 25% agarose | $424.00 | |||
TRAP-α Antibody (C-8) HRP | sc-373916 HRP | 200 µg/ml | $322.00 | |||
TRAP-α Antibody (C-8) FITC | sc-373916 FITC | 200 µg/ml | $336.00 | |||
TRAP-α Antibody (C-8) PE | sc-373916 PE | 200 µg/ml | $349.00 | |||
TRAP-α Antibody (C-8) Alexa Fluor® 488 | sc-373916 AF488 | 200 µg/ml | $364.00 | |||
TRAP-α Antibody (C-8) Alexa Fluor® 546 | sc-373916 AF546 | 200 µg/ml | $364.00 | |||
TRAP-α Antibody (C-8) Alexa Fluor® 594 | sc-373916 AF594 | 200 µg/ml | $364.00 | |||
TRAP-α Antibody (C-8) Alexa Fluor® 647 | sc-373916 AF647 | 200 µg/ml | $364.00 | |||
TRAP-α Antibody (C-8) Alexa Fluor® 680 | sc-373916 AF680 | 200 µg/ml | $364.00 | |||
TRAP-α Antibody (C-8) Alexa Fluor® 790 | sc-373916 AF790 | 200 µg/ml | $364.00 |