TMEM125 CRISPR/Cas9 KO Plasmid (h): sc-414318
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TMEM125 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TMEM125 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TMEM125 CRISPR/Cas9 KO Plasmid (h) | sc-414318 | 20 µg | $397.00 |
Overview
TMEM125 encodes a multi-pass transmembrane protein with emerging roles in membrane organization and intracellular trafficking, processes that influence receptor turnover and compartmentalized signaling. Its expression has been reported in multiple tissues, and transcript-level dysregulation has been observed in several disease datasets, suggesting potential relevance to cellular homeostasis under stress. Although mechanistic annotation remains limited, TMEM125 is frequently evaluated in functional genomics efforts focused on membrane-proximal pathways, organelle dynamics, and proteostasis. These features make TMEM125 a useful target for dissecting how membrane proteins shape signaling networks and cell-state transitions.
TMEM125 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TMEM125 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TMEM125 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TMEM125 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TMEM125 protein expression.
This CRISPR knockout system enables efficient generation of TMEM125-deficient cell models for investigation of TMEM125 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TMEM125 exon(s) critical for TMEM125 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TMEM125 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TMEM125 CRISPR/Cas9 KO Plasmid (h) and TMEM125 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TMEM125 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TMEM125 HDR Plasmid (h) and TMEM125 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TMEM125 homology arms to support homology-directed repair at defined TMEM125 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.