TM4SF19 CRISPR/Cas9 KO Plasmid (h): sc-406884
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TM4SF19 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TM4SF19 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TM4SF19 CRISPR/Cas9 KO Plasmid (h) | sc-406884 | 20 µg | $397.00 |
Overview
TM4SF19 (transmembrane 4 L six family member 19) encodes a tetraspan-like membrane protein predicted to organize membrane microdomains and modulate cell–cell or cell–matrix interactions. As a member of the TM4SF family, TM4SF19 is linked to processes such as receptor clustering, signal transduction at the plasma membrane, and regulation of adhesion-associated pathways that influence migration and epithelial organization. Altered expression of TM4SF proteins has been reported across multiple cancer contexts, supporting investigation of TM4SF19 in tumor biology, invasion phenotypes, and microenvironmental signaling. TM4SF19 perturbation studies can also inform mechanisms of membrane protein trafficking and surfaceome composition in human cells.
TM4SF19 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TM4SF19 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TM4SF19 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TM4SF19 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TM4SF19 protein expression.
This CRISPR knockout system enables efficient generation of TM4SF19-deficient cell models for investigation of TM4SF19 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TM4SF19 exon(s) critical for TM4SF19 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TM4SF19 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TM4SF19 CRISPR/Cas9 KO Plasmid (h) and TM4SF19 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TM4SF19 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TM4SF19 HDR Plasmid (h) and TM4SF19 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TM4SF19 homology arms to support homology-directed repair at defined TM4SF19 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.