Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TFIIIB′′ CRISPR/Cas9 KO Plasmid (m): sc-436781
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TFIIIB″ CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TFIIIB″ genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: BDP1 Antibody (B-6): sc-515058
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TFIIIB′′ CRISPR/Cas9 KO Plasmid (m) | sc-436781 | 20 µg | $397.00 |
Overview
Bdp1 encodes the mouse TFIIIB″ subunit, a core component of the RNA polymerase III transcription machinery that cooperates with TBP and BRF proteins to assemble TFIIIB at Pol III promoters. TFIIIB″ supports initiation and reinitiation of Pol III–dependent transcription of tRNAs, 5S rRNA, and other small noncoding RNAs, linking Bdp1 activity to translational capacity, proteostasis, and cellular growth control. Because Pol III output is tightly coupled to nutrient sensing and stress responses, perturbation of Bdp1 can influence cell-cycle progression and metabolic adaptation. Dysregulated Pol III transcription and altered TFIIIB function are frequently studied in contexts of proliferative disorders and genome stability pathways, making Bdp1 a useful target for mechanistic studies in mammalian cells.
TFIIIB″ CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Bdp1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Bdp1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Bdp1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TFIIIB″ protein expression.
This CRISPR knockout system enables efficient generation of Bdp1-deficient cell models for investigation of TFIIIB″ signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Bdp1 exon(s) critical for TFIIIB″ function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Bdp1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TFIIIB″ CRISPR/Cas9 KO Plasmid (m) and TFIIIB″ CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Bdp1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TFIIIB″ HDR Plasmid (m) and TFIIIB″ HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Bdp1 homology arms to support homology-directed repair at defined Bdp1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.