Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Testican-1 CRISPR/Cas9 KO Plasmid (h): sc-406915
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Testican-1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Testican-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Testican-1 Antibody (C-3): sc-390789
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Testican-1 CRISPR/Cas9 KO Plasmid (h) | sc-406915 | 20 µg | $397.00 |
Overview
SPOCK1 encodes testican-1, a secreted extracellular matrix proteoglycan in the SPARC family that modulates cell–matrix interactions and pericellular proteolysis. Testican-1 can influence extracellular remodeling by interacting with heparan/chondroitin sulfate components and regulating protease activity, thereby affecting cell adhesion, migration, and tissue organization. Through these extracellular matrix–dependent processes, SPOCK1 has been studied in contexts of invasive cell behavior, stromal remodeling, and inflammatory microenvironments. Dysregulated SPOCK1 expression has been reported across multiple tumor types and fibrotic or remodeling-associated pathologies, supporting its use as a mechanistic node for investigating microenvironment-driven phenotypes.
Testican-1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SPOCK1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SPOCK1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SPOCK1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Testican-1 protein expression.
This CRISPR knockout system enables efficient generation of SPOCK1-deficient cell models for investigation of Testican-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SPOCK1 exon(s) critical for Testican-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SPOCK1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Testican-1 CRISPR/Cas9 KO Plasmid (h) and Testican-1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SPOCK1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Testican-1 HDR Plasmid (h) and Testican-1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SPOCK1 homology arms to support homology-directed repair at defined SPOCK1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.