Svs6 CRISPR/Cas9 KO Plasmid (m): sc-423228
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Svs6 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Svs6 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Svs6 CRISPR/Cas9 KO Plasmid (m) | sc-423228 | 20 µg | $397.00 |
Overview
Mouse Svs6 encodes seminal vesicle secretory protein 6, a male reproductive tract protein that contributes to the composition and biophysical properties of seminal fluid. Svs6 is part of the androgen-responsive secretory program in seminal vesicle epithelium and is commonly used as a marker of accessory gland differentiation and luminal secretory activity. By influencing seminal plasma milieu, Svs6 is relevant to processes that impact sperm survival, motility, and post-ejaculatory reproductive tract interactions. Altered expression of seminal vesicle secretory proteins is frequently examined in models of endocrine disruption, inflammation, and reproductive phenotypes, supporting its utility in pathway-level studies of male fertility and accessory gland biology.
Svs6 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Svs6 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Svs6 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Svs6 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Svs6 protein expression.
This CRISPR knockout system enables efficient generation of Svs6-deficient cell models for investigation of Svs6 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Svs6 exon(s) critical for Svs6 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Svs6 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Svs6 CRISPR/Cas9 KO Plasmid (m) and Svs6 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Svs6 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Svs6 HDR Plasmid (m) and Svs6 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Svs6 homology arms to support homology-directed repair at defined Svs6 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.