SUR-2 CRISPR/Cas9 KO Plasmid (m): sc-423218

Overview

Abcc9 encodes sulfonylurea receptor 2 (SUR-2), a regulatory subunit of ATP-sensitive potassium (KATP) channels that couples cellular metabolic status to membrane excitability by controlling Kir6.x channel gating. In mouse, SUR-2–containing KATP channels are prominent in cardiac and vascular smooth muscle, where they shape action potential duration, calcium handling, and vascular tone in response to shifts in ATP/ADP and phospholipid signaling. Through these roles, ABCC9 integrates bioenergetic cues with stress-adaptive pathways such as ischemic preconditioning, smooth muscle contractility programs, and mitochondrial function. Dysregulation of ABCC9/KATP channel activity has been linked to cardiovascular and metabolic phenotypes, and human ABCC9 variants are associated with channelopathy syndromes affecting heart and vasculature, making Abcc9 a relevant target for mechanistic disease modeling.

SUR-2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Abcc9 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Abcc9 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Abcc9 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SUR-2 protein expression.

This CRISPR knockout system enables efficient generation of Abcc9-deficient cell models for investigation of SUR-2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting Abcc9 exon(s) critical for SUR-2 function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple Abcc9 genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by SUR-2 CRISPR/Cas9 KO Plasmid (m) and SUR-2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Abcc9 locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by SUR-2 HDR Plasmid (m) and SUR-2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Abcc9 homology arms to support homology-directed repair at defined Abcc9 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.