SMO/Smoothened Antibody (E-5): sc-166685

Smo Antibody (E-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects Smo protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Smo antibody (E-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Smo protein plays a critical role in the Hedgehog signaling pathway, which regulates cell growth, differentiation, and tissue patterning during embryonic development. Aberrant activation of this pathway, often due to mutations in Smo gene or regulatory components, has been implicated in various cancers, making Smo protein a significant target for therapeutic intervention. Smo (E-5) antibody recognizes a seven transmembrane receptor that transmits signals from Hedgehog ligand to downstream effectors, including GLI transcription factors, thereby influencing gene expression and cellular responses. Understanding Smo protein structure and function is vital, as Smo protein mediates Hedgehog signaling effects and interacts with other pathway proteins, such as Patched, which serves as a negative regulator. The balance between these proteins is crucial for normal development and prevention of tumorigenesis, highlighting Smo protein′s importance in both developmental biology and cancer research.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

SMO/Smoothened Antibody (E-5) References:

1. Genomic analysis of non-NF2 meningiomas reveals mutations in TRAF7, KLF4, AKT1, and SMO.  |  Clark, VE., et al. 2013. Science. 339: 1077-80. PMID: 23348505
2. Fibroblast growth factor 18 attenuates liver fibrosis and HSCs activation via the SMO-LATS1-YAP pathway.  |  Tong, G., et al. 2022. Pharmacol Res. 178: 106139. PMID: 35202822
3. Identification of an amplified, highly expressed gene in a human glioma.  |  Kinzler, KW., et al. 1987. Science. 236: 70-3. PMID: 3563490
4. The role of Smo-Shh/Gli signaling activation in the prevention of neurological and ageing disorders.  |  Prajapati, A., et al. 2023. Biogerontology. 24: 493-531. PMID: 37097427
5. Human umbilical cord mesenchymal stem cell-derived exosomes ameliorate renal fibrosis in diabetic nephropathy by targeting Hedgehog/SMO signaling.  |  Zhang, K., et al. 2024. FASEB J. 38: e23599. PMID: 38572590
6. Activation of Shh/Smo is sufficient to maintain oligodendrocyte precursor cells in an undifferentiated state and is not necessary for myelin formation and (re)myelination.  |  Nocera, S., et al. 2024. Glia. 72: 1469-1483. PMID: 38771121
7. Mutations affecting segment number and polarity in Drosophila.  |  Nüsslein-Volhard, C. and Wieschaus, E. 1980. Nature. 287: 795-801. PMID: 6776413
8. Floor plate and motor neuron induction by different concentrations of the amino-terminal cleavage product of sonic hedgehog autoproteolysis.  |  Roelink, H., et al. 1995. Cell. 81: 445-55. PMID: 7736596
9. Requirement of 19K form of Sonic hedgehog for induction of distinct ventral cell types in CNS explants.  |  Martí, E., et al. 1995. Nature. 375: 322-5. PMID: 7753196
10. Activity of Wnt-1 as a transmembrane protein.  |  Parkin, NT., et al. 1993. Genes Dev. 7: 2181-93. PMID: 8224845
11. Human homolog of patched, a candidate gene for the basal cell nevus syndrome.  |  Johnson, RL., et al. 1996. Science. 272: 1668-71. PMID: 8658145
12. The tumour-suppressor gene patched encodes a candidate receptor for Sonic hedgehog.  |  Stone, DM., et al. 1996. Nature. 384: 129-34. PMID: 8906787

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SMO/Smoothened Antibody (E-5) has 97 citations in a variety of scientific publications.

Primary antibodies like Anti-SMO/Smoothened Antibody (E-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SMO/Smoothened Antibody (E-5) Specifications

Alternate Product Name: Smoothened

Product Clone Name: E-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 488-787

Epitope Target: Smo

Epitope Species: human

Product Reactivity/Detection: Smo

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 97 citations in various scientific publications

Available Conjugates/Forms for SMO/Smoothened Antibody (E-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, B: Biotin, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin