Slit2 Lentiviral Activation Particles (h): sc-401586-LAC

SLIT2 encodes Slit2, a secreted extracellular matrix–associated guidance cue that binds ROBO receptors to regulate axon pathfinding, neuronal migration, and directional cell motility. Beyond neurodevelopment, Slit2–ROBO signaling influences cytoskeletal dynamics through Rho family GTPases and intersects with pathways controlling adhesion and chemotaxis, including modulation of CXCL12/CXCR4-driven migration in some contexts. Altered SLIT2 expression and epigenetic silencing have been reported across multiple tumor types and are frequently studied for roles in invasion, angiogenesis, and metastatic behavior. SLIT2 is also investigated in vascular biology and inflammatory processes where guidance cues shape endothelial and immune cell trafficking.

Slit2 Lentiviral Activation Particles (h) address this need by packaging the complete synergistic activation mediator (SAM) transcriptional activation system into transduction-ready, high-titer lentiviral particles, enabling efficient SLIT2 upregulation across a broader range of human cell types.

Slit2 Lentiviral Activation Particles (h) deliver all functional components of the synergistic activation mediator (SAM) system via lentiviral transduction. The system comprises three particle preparations co-transduced into target cells: one encoding catalytically inactive dCas9 (D10A and N863A mutations) fused to the VP64 transactivation domain with a blasticidin resistance gene; one encoding the MS2-p65-HSF1 fusion protein with a hygromycin resistance gene; and one encoding a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers with a puromycin resistance gene. Following lentiviral transduction and genomic integration of the expression cassettes, the SAM components are stably expressed and assemble at the target locus within the proximal promoter region upstream of the SLIT2 transcriptional start site, where VP64, p65, and HSF1 act cooperatively to recruit endogenous transcriptional machinery and drive sustained upregulation of endogenous Slit2 expression. The use of nuclease-inactive dCas9 avoids the introduction of double-strand DNA breaks and preserves the native SLIT2 genomic locus and regulatory architecture.

The lentiviral format offers several practical advantages: stable genomic integration supports heritable activation across cell divisions; high-titer particle preparations eliminate the need for in-house viral production; and compatibility with primary, non-dividing, and transfection-resistant cell types expands experimental accessibility. Successful transduction can be confirmed and enriched through triple antibiotic selection using puromycin, hygromycin, and blasticidin.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.