Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SKIP CRISPR/Cas9 KO Plasmid (h): sc-407373
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SKIP CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SKIP genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SKIP Antibody (B-6): sc-365362
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SKIP CRISPR/Cas9 KO Plasmid (h) | sc-407373 | 20 µg | $397.00 |
Overview
INPP5K encodes the inositol polyphosphate-5-phosphatase K, commonly referred to as SKIP, a phosphoinositide phosphatase that regulates cellular phosphoinositol signaling by dephosphorylating PtdIns(4,5)P₂ and related substrates. By tuning phosphoinositide composition at membranes, SKIP influences actin cytoskeleton organization, membrane trafficking, and signal transduction downstream of pathways such as PI3K/AKT that depend on localized lipid second messengers. Altered INPP5K activity has been linked to disruptions in muscle and neurodevelopmental homeostasis, making it relevant to studies of congenital disorders affecting neuromuscular function and cellular architecture. Because phosphoinositides coordinate multiple compartments and processes, SKIP serves as a useful node for dissecting membrane–cytoskeleton coupling and phosphatase-controlled signaling dynamics in human cells.
SKIP CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the INPP5K gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the INPP5K together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the INPP5K open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SKIP protein expression.
This CRISPR knockout system enables efficient generation of INPP5K-deficient cell models for investigation of SKIP signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting INPP5K exon(s) critical for SKIP function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple INPP5K genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SKIP CRISPR/Cas9 KO Plasmid (h) and SKIP CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the INPP5K locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SKIP HDR Plasmid (h) and SKIP HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by INPP5K homology arms to support homology-directed repair at defined INPP5K target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.