SIRP-γ Antibody (OX119): sc-53114. Indirect FCM analysis of human peripheral blood leukocytes stained with SIRP-γ Antibody (OX119), followed by PE-conjugated goat anti-mouse IgG1: sc-3764. Black line histogram represents the isotype control, normal mouse IgG1: sc-3877.
SIRP-γ Antibody (OX119): sc-53114
- SIRP-γ Antibody (OX119) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- raised against recombinant SIRP-γ of human origin
- recommended for detection of SIRP-γ of human origin by WB, IP and FCM; may cross-react with over-expressed SIRP-α and SIRP-β
- Anti-SIRP-gamma Antibody (OX119) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for SIRP-γ Antibody (OX119) for WB applications. These reagents are now offered in bundles with SIRP-γ Antibody (OX119) (see ordering information below).
SIRP-γ Antibody (OX119) is a mouse monoclonal IgG1 antibody that detects SIRP-γ in human samples through applications such as western blotting (WB), immunoprecipitation (IP), and flow cytometry (FCM). SIRP-γ, also known as CD172g, is a transmembrane glycoprotein that plays a crucial role in immune regulation, particularly in T cells and a subset of B cells. SIRP-γ unique structure includes an extracellular domain with immunoglobulin-like motifs, which facilitates interaction with CD47, a well-known "don′t eat me" signal that inhibits phagocytosis by macrophages. This interaction is vital for maintaining immune homeostasis and preventing autoimmunity, as SIRP-γ modulates immune response by signaling to immune cells when to refrain from attacking. The importance of SIRP-γ in immune regulation highlights potential as a therapeutic target in various diseases, including cancer and autoimmune disorders. Anti-SIRP-γ antibody (OX119) is an essential tool for researchers aiming to explore the intricate mechanisms of immune signaling and SIRP-γ role in health and disease.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SIRP-γ Antibody (OX119) References:
- Inhibition of EGFR-mediated phosphoinositide-3-OH kinase (PI3-K) signaling and glioblastoma phenotype by signal-regulatory proteins (SIRPs). | Wu, CJ., et al. 2000. Oncogene. 19: 3999-4010. PMID: 10962556
- Bidirectional negative regulation of human T and dendritic cells by CD47 and its cognate receptor signal-regulator protein-alpha: down-regulation of IL-12 responsiveness and inhibition of dendritic cell activation. | Latour, S., et al. 2001. J Immunol. 167: 2547-54. PMID: 11509594
- Human lymphocytes interact directly with CD47 through a novel member of the signal regulatory protein (SIRP) family. | Brooke, G., et al. 2004. J Immunol. 173: 2562-70. PMID: 15294972
- Transcriptional regulation of signal regulatory protein alpha1 inhibitory receptors by epidermal growth factor receptor signaling. | Kapoor, GS., et al. 2004. Cancer Res. 64: 6444-52. PMID: 15374953
- SIRPbeta1 is expressed as a disulfide-linked homodimer in leukocytes and positively regulates neutrophil transepithelial migration. | Liu, Y., et al. 2005. J Biol Chem. 280: 36132-40. PMID: 16081415
- Identification of the major SHPTP2-binding protein that is tyrosine-phosphorylated in response to insulin. | Yamauchi, K., et al. 1995. J Biol Chem. 270: 17716-22. PMID: 7629070
- A novel membrane glycoprotein, SHPS-1, that binds the SH2-domain-containing protein tyrosine phosphatase SHP-2 in response to mitogens and cell adhesion. | Fujioka, Y., et al. 1996. Mol Cell Biol. 16: 6887-99. PMID: 8943344
- A family of proteins that inhibit signalling through tyrosine kinase receptors. | Kharitonenkov, A., et al. 1997. Nature. 386: 181-6. PMID: 9062191
- Growth hormone regulation of SIRP and SHP-2 tyrosyl phosphorylation and association. | Stofega, MR., et al. 1998. J Biol Chem. 273: 7112-7. PMID: 9507023
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SIRP-γ Antibody (OX119) | sc-53114 | 200 µg/ml | $322.00 | |||
SIRP-γ Antibody (OX119): m-IgG Fc BP-HRP Bundle | sc-536892 | 200 µg Ab; 10 µg BP | $361.00 | |||
SIRP-γ Antibody (OX119): m-IgGκ BP-HRP Bundle | sc-534064 | 200 µg Ab; 40 µg BP | $361.00 | |||
SIRP-γ Antibody (OX119): m-IgG1 BP-HRP Bundle | sc-544882 | 200 µg Ab; 20 µg BP | $361.00 | |||
SIRP-γ Antibody (OX119) AC | sc-53114 AC | 500 µg/ml, 25% agarose | $424.00 | |||
SIRP-γ Antibody (OX119) HRP | sc-53114 HRP | 200 µg/ml | $322.00 | |||
SIRP-γ Antibody (OX119) FITC | sc-53114 FITC | 200 µg/ml | $336.00 | |||
SIRP-γ Antibody (OX119) PE | sc-53114 PE | 200 µg/ml | $349.00 | |||
SIRP-γ Antibody (OX119) Alexa Fluor® 488 | sc-53114 AF488 | 200 µg/ml | $364.00 | |||
SIRP-γ Antibody (OX119) Alexa Fluor® 546 | sc-53114 AF546 | 200 µg/ml | $364.00 | |||
SIRP-γ Antibody (OX119) Alexa Fluor® 594 | sc-53114 AF594 | 200 µg/ml | $364.00 | |||
SIRP-γ Antibody (OX119) Alexa Fluor® 647 | sc-53114 AF647 | 200 µg/ml | $364.00 | |||
SIRP-γ Antibody (OX119) Alexa Fluor® 680 | sc-53114 AF680 | 200 µg/ml | $364.00 | |||
SIRP-γ Antibody (OX119) Alexa Fluor® 790 | sc-53114 AF790 | 200 µg/ml | $364.00 |