SEZ6 CRISPR/Cas9 KO Plasmid (m): sc-422895
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SEZ6 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SEZ6 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SEZ6 CRISPR/Cas9 KO Plasmid (m) | sc-422895 | 20 µg | $397.00 |
Overview
Sez6 encodes seizure-related 6 homolog (SEZ6), a type I transmembrane protein enriched in the nervous system that contributes to neuronal differentiation, dendritic arborization, and synapse formation. SEZ6 participates in membrane trafficking and proteolytic processing pathways, including ectodomain shedding that can influence cell–cell signaling and neurite outgrowth programs. In mouse models, altered Sez6 expression or function has been linked to changes in synaptic connectivity and excitatory/inhibitory balance, supporting its relevance to neurodevelopmental and seizure-related phenotypes. As a neuronal surface-associated regulator, SEZ6 is frequently studied in the context of circuit maturation and activity-dependent plasticity.
SEZ6 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Sez6 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Sez6 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Sez6 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SEZ6 protein expression.
This CRISPR knockout system enables efficient generation of Sez6-deficient cell models for investigation of SEZ6 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Sez6 exon(s) critical for SEZ6 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Sez6 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SEZ6 CRISPR/Cas9 KO Plasmid (m) and SEZ6 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Sez6 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SEZ6 HDR Plasmid (m) and SEZ6 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Sez6 homology arms to support homology-directed repair at defined Sez6 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.