Scrib CRISPR/Cas9 KO Plasmid (m): sc-430616
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Scrib CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Scrib genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Scrib Antibody (D-2): sc-374139
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Scrib CRISPR/Cas9 KO Plasmid (m) | sc-430616 | 20 µg | $397.00 |
Overview
Scrib encodes Scribble, a conserved polarity scaffold protein that localizes to cell–cell junctions and coordinates apical–basal polarity, epithelial architecture, and directed cell migration. In mouse systems, Scribble integrates signals from polarity networks with junctional and cytoskeletal regulators, influencing processes such as planar cell polarity, vesicle trafficking, and tissue morphogenesis. Disruption of SCRIB-dependent polarity control is associated with altered proliferation and invasiveness, linking this pathway to developmental abnormalities and cancer-relevant phenotypes. Scrib is therefore widely studied in contexts where junction integrity, migration, and polarity-dependent signaling shape tissue organization and disease mechanisms.
Scrib CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Scrib gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Scrib together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Scrib open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Scrib protein expression.
This CRISPR knockout system enables efficient generation of Scrib-deficient cell models for investigation of Scrib signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Scrib exon(s) critical for Scrib function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Scrib genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Scrib CRISPR/Cas9 KO Plasmid (m) and Scrib CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Scrib locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Scrib HDR Plasmid (m) and Scrib HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Scrib homology arms to support homology-directed repair at defined Scrib target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.