Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SCML1 CRISPR/Cas9 KO Plasmid (h): sc-407161
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SCML1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SCML1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SCML1 Antibody (8-RY28): sc-135622
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SCML1 CRISPR/Cas9 KO Plasmid (h) | sc-407161 | 20 µg | $397.00 |
Overview
SCML1 (sex comb on midleg-like 1) encodes a Polycomb group–associated protein implicated in chromatin-dependent transcriptional repression and maintenance of epigenetic states. As a member of the SCM family, SCML1 is linked to Polycomb repressive complex biology, contributing to histone-mark–guided regulation of gene expression programs that influence cell identity and developmental processes. Altered Polycomb pathway activity is broadly relevant to dysregulated differentiation and genome-wide transcriptional imbalance observed in multiple disease contexts, making SCML1 a useful node for studying epigenetic control mechanisms. Investigation of SCML1 supports research into chromatin organization, transcriptional silencing, and downstream effects on lineage-specific gene networks.
SCML1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SCML1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SCML1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SCML1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SCML1 protein expression.
This CRISPR knockout system enables efficient generation of SCML1-deficient cell models for investigation of SCML1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SCML1 exon(s) critical for SCML1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SCML1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SCML1 CRISPR/Cas9 KO Plasmid (h) and SCML1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SCML1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SCML1 HDR Plasmid (h) and SCML1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SCML1 homology arms to support homology-directed repair at defined SCML1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.