Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SAP 49 CRISPR/Cas9 KO Plasmid (h2): sc-407057-KO-2
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SAP 49 CRISPR/Cas9 Knockout (KO) Plasmid (h2) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SAP 49 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SAP 49 Antibody (G-12): sc-365570
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SAP 49 CRISPR/Cas9 KO Plasmid (h2) | sc-407057-KO-2 | 20 µg | $397.00 |
Overview
SF3B4 encodes splicing factor 3B subunit 4 (SAP 49), a core component of the U2 snRNP within the SF3b complex that promotes accurate recognition of the branch point and 3′ splice site during pre-mRNA splicing. By helping assemble and stabilize spliceosome A and B complexes, SAP 49 influences transcript isoform selection and supports coordinated gene expression programs linked to cell-cycle progression, DNA damage responses, and differentiation. Disruption of spliceosomal fidelity can drive widespread alternative splicing changes and proteome remodeling, making SF3B4 a useful node for studying RNA processing networks in human cells. Altered SF3B4 expression and spliceosome dysfunction have been associated with pathological states in which aberrant splicing contributes to cellular stress responses and dysregulated growth.
SAP 49 CRISPR/Cas9 KO Plasmid (h2) is a pool of plasmids designed for targeted disruption of the SF3B4 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SF3B4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SF3B4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SAP 49 protein expression.
This CRISPR knockout system enables efficient generation of SF3B4-deficient cell models for investigation of SAP 49 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SF3B4 exon(s) critical for SAP 49 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SF3B4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SAP 49 CRISPR/Cas9 KO Plasmid (h) and SAP 49 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SF3B4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SAP 49 HDR Plasmid (h) and SAP 49 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SF3B4 homology arms to support homology-directed repair at defined SF3B4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.