RTP4 CRISPR/Cas9 KO Plasmid (m): sc-426736
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RTP4 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the RTP4 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RTP4 CRISPR/Cas9 KO Plasmid (m) | sc-426736 | 20 µg | $397.00 |
Overview
Receptor transporter protein 4 (RTP4) is a member of the RTP family implicated in intracellular trafficking and functional expression of select G protein-coupled receptors (GPCRs), influencing receptor maturation, membrane localization, and signal responsiveness. In mouse, Rtp4 expression is commonly associated with interferon-stimulated gene programs and innate immune signaling, linking it to regulation of antiviral responses and inflammatory transcriptional states. Through effects on receptor processing and immune-associated pathways, RTP4 can shape cellular responses to extracellular cues and cytokine environments. Dysregulated RTP4-related signaling and interferon pathway activation are frequently studied in contexts such as neuroimmune interactions, infection models, and inflammation-associated phenotypes.
RTP4 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Rtp4 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Rtp4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Rtp4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish RTP4 protein expression.
This CRISPR knockout system enables efficient generation of Rtp4-deficient cell models for investigation of RTP4 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Rtp4 exon(s) critical for RTP4 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Rtp4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by RTP4 CRISPR/Cas9 KO Plasmid (m) and RTP4 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Rtp4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by RTP4 HDR Plasmid (m) and RTP4 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Rtp4 homology arms to support homology-directed repair at defined Rtp4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.