Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ROR2 CRISPR/Cas9 KO Plasmid (m): sc-424100
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ROR2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ROR2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ROR2 Antibody (H-1): sc-374174
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ROR2 CRISPR/Cas9 KO Plasmid (m) | sc-424100 | 20 µg | $397.00 |
Overview
Ror2 encodes the receptor tyrosine kinase-like orphan receptor 2 (ROR2), a membrane receptor that functions primarily as a co-receptor in non-canonical WNT signaling, particularly in response to WNT5A. ROR2 regulates planar cell polarity, cytoskeletal dynamics, and directed cell migration through downstream pathways that include JNK/AP-1 and Rho-family GTPase signaling. In mouse developmental biology, Ror2 activity influences skeletal morphogenesis, limb patterning, and tissue organization, making it a key node in studies of differentiation and morphogenetic signaling. Dysregulated ROR2-associated signaling has been linked to aberrant cell motility and invasive phenotypes in disease-relevant contexts, supporting its use as a mechanistic handle in pathway and phenotype interrogation.
ROR2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ror2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ror2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ror2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ROR2 protein expression.
This CRISPR knockout system enables efficient generation of Ror2-deficient cell models for investigation of ROR2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ror2 exon(s) critical for ROR2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ror2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ROR2 CRISPR/Cas9 KO Plasmid (m) and ROR2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ror2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ROR2 HDR Plasmid (m) and ROR2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ror2 homology arms to support homology-directed repair at defined Ror2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.