PRODH2 CRISPR/Cas9 KO Plasmid (h2): sc-405889-KO-2
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PRODH2 CRISPR/Cas9 Knockout (KO) Plasmid (h2) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PRODH2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PRODH2 CRISPR/Cas9 KO Plasmid (h2) | sc-405889-KO-2 | 20 µg | $397.00 |
Overview
PRODH2 encodes a mitochondrial flavin-dependent oxidoreductase that participates in proline and hydroxyproline catabolism, linking amino acid turnover to cellular redox balance and energy metabolism. By coupling electron transfer reactions to the respiratory chain, PRODH2 activity can influence reactive oxygen species homeostasis and metabolic adaptation under stress. Dysregulation of proline metabolism has been associated with altered mitochondrial function and redox signaling observed in a range of neurodevelopmental and metabolic phenotypes. As a result, PRODH2 is studied in pathways connecting mitochondrial metabolism, amino acid utilization, and oxidative stress responses.
PRODH2 CRISPR/Cas9 KO Plasmid (h2) is a pool of plasmids designed for targeted disruption of the PRODH2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PRODH2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PRODH2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PRODH2 protein expression.
This CRISPR knockout system enables efficient generation of PRODH2-deficient cell models for investigation of PRODH2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PRODH2 exon(s) critical for PRODH2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PRODH2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PRODH2 CRISPR/Cas9 KO Plasmid (h) and PRODH2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PRODH2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PRODH2 HDR Plasmid (h) and PRODH2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PRODH2 homology arms to support homology-directed repair at defined PRODH2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.