PNP Antibody (D-11): sc-271890
- PNP Antibody (D-11) is a mouse monoclonal IgG1, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 95-150 mapping within an internal region of PNP of human origin
- recommended for detection of PNP of mouse, rat, human origin by WB, IP, IF and ELISA
- m-IgG1 BP-HRP is the preferred secondary detection reagent for PNP Antibody (D-11) for WB applications. This reagent is now offered in a bundle with PNP Antibody (D-11) (see ordering information below).
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PNP Antibody (D-11) is a mouse monoclonal IgG1 antibody that detects purine nucleoside phosphorylase (PNP) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-PNP antibody (D-11) recognizes PNP, which plays a crucial role in the purine salvage pathway by catalyzing the reversible phosphorolysis of ribonucleosides and 2′-deoxyribonucleosides, specifically targeting guanine and hypoxanthine. This enzymatic activity is vital for maintaining nucleotide balance within cells, particularly in lymphocytes, where PNP is essential for proper immune function. Deficiencies in PNP can lead to severe immunodeficiency and neurological disorders, highlighting the importance of PNP in both the immune system and overall cellular health. PNP forms a homotrimer, which is significant for stability and function, as the trimeric structure enhances enzymatic efficiency and regulatory mechanisms. PNP monoclonal antibody (D-11) is an invaluable tool for researchers studying the implications of PNP in various diseases, including combined immunodeficiency and potential links to autoimmune disorders and cancer.
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PNP Antibody (D-11) References:
- Crystal structure of human PNP complexed with guanine. | de Azevedo, WF., et al. 2003. Biochem Biophys Res Commun. 312: 767-72. PMID: 14680831
- Structure of human PNP complexed with ligands. | Canduri, F., et al. 2005. Acta Crystallogr D Biol Crystallogr. 61: 856-62. PMID: 15983407
- Tryptophan-free human PNP reveals catalytic site interactions. | Ghanem, M., et al. 2008. Biochemistry. 47: 3202-15. PMID: 18269249
- The transition state analog inhibitor of Purine Nucleoside Phosphorylase (PNP) Immucillin-H arrests bone loss in rat periodontal disease models. | Deves, C., et al. 2013. Bone. 52: 167-75. PMID: 23026564
- Evidence for purine nucleoside phosphorylase (PNP) release from rat C6 glioma cells. | Giuliani, P., et al. 2017. J Neurochem. 141: 208-221. PMID: 28251649
- PNP inhibitors selectively kill cancer cells lacking SAMHD1. | Davenne, T. and Rehwinkel, J. 2020. Mol Cell Oncol. 7: 1804308. PMID: 33235905
- Intratumoral immunotherapy using platelet-cloaked nanoparticles enhances antitumor immunity in solid tumors. | Bahmani, B., et al. 2021. Nat Commun. 12: 1999. PMID: 33790276
- Cryo-EM structure of AMP-PNP-bound human mitochondrial ATP-binding cassette transporter ABCB7. | Yan, Q., et al. 2022. J Struct Biol. 214: 107832. PMID: 35041979
- 'PNP slows down' - linearly-reduced whole body joint velocities and altered gait patterns in polyneuropathy. | Walz, ID., et al. 2023. Front Hum Neurosci. 17: 1229440. PMID: 37780958
- Human induced pluripotent stem cell/embryonic stem cell-derived pyramidal neuronal precursors show safety and efficacy in a rat spinal cord injury model. | Li, M., et al. 2024. Cell Mol Life Sci. 81: 318. PMID: 39073571
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PNP Antibody (D-11) | sc-271890 | 200 µg/ml | $322.00 | |||
PNP Antibody (D-11): m-IgG1 BP-HRP Bundle | sc-542684 | 200 µg Ab; 20 µg BP | $361.00 |