PHT2 CRISPR/Cas9 KO Plasmid (m): sc-425761
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PHT2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PHT2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PHT2 CRISPR/Cas9 KO Plasmid (m) | sc-425761 | 20 µg | $397.00 |
Overview
Slc15a3 encodes the proton-coupled histidine/dipeptide transporter PHT2, a member of the SLC15 family implicated in endolysosomal solute transport and intracellular peptide handling. By coupling peptide transport to proton gradients, PHT2 contributes to lysosomal homeostasis, nutrient sensing, and crosstalk between vesicular trafficking and metabolic programs in myeloid and other immune-relevant cell types. Altered SLC15-family activity has been linked to dysregulated innate immune signaling and inflammatory states, making Slc15a3 a useful node for studying how endosomal/lysosomal transport influences cytokine responses and antigen-processing-associated pathways. In mouse systems, Slc15a3 perturbation supports mechanistic interrogation of vesicular pH-dependent processes that shape immune activation and tissue inflammation models.
PHT2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Slc15a3 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Slc15a3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Slc15a3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PHT2 protein expression.
This CRISPR knockout system enables efficient generation of Slc15a3-deficient cell models for investigation of PHT2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Slc15a3 exon(s) critical for PHT2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Slc15a3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PHT2 CRISPR/Cas9 KO Plasmid (m) and PHT2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Slc15a3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PHT2 HDR Plasmid (m) and PHT2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Slc15a3 homology arms to support homology-directed repair at defined Slc15a3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.