PHF3 CRISPR/Cas9 KO Plasmid (h): sc-406857
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PHF3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PHF3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PHF3 CRISPR/Cas9 KO Plasmid (h) | sc-406857 | 20 µg | $397.00 |
Overview
PHF3 (PHD finger protein 3) is a nuclear transcriptional regulator that associates with RNA polymerase II and contributes to control of transcription elongation and co-transcriptional RNA processing. Through its PHD finger domains and interactions with elongation machinery, PHF3 influences gene expression programs linked to cell-state maintenance and stimulus-responsive transcription. Perturbation of PHF3 activity has been connected to altered transcriptional fidelity and dysregulated growth-control networks, making it relevant for studying mechanisms that couple chromatin-associated factors to RNA synthesis. PHF3 is therefore of interest in research areas spanning gene regulation, differentiation, and disease-associated transcriptional rewiring.
PHF3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PHF3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PHF3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PHF3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PHF3 protein expression.
This CRISPR knockout system enables efficient generation of PHF3-deficient cell models for investigation of PHF3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PHF3 exon(s) critical for PHF3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PHF3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PHF3 CRISPR/Cas9 KO Plasmid (h) and PHF3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PHF3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PHF3 HDR Plasmid (h) and PHF3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PHF3 homology arms to support homology-directed repair at defined PHF3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.